Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DSHH00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (34 µL), Serum (34 µL), Heparin Plasma (34 µL)
Sensitivity
3.92 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Product Summary for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
The Quantikine Human Sonic Hedgehog/Shh N-Terminus Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Shh-N in cell culture supernates, serum, and heparin plasma. It contains E. coli-expressed recombinant human Shh-N and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Shh-N showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Shh-N.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 99.5 | 291 | 532 | 109 | 318 | 612 |
| Standard Deviation | 5.97 | 8.15 | 14.4 | 10.3 | 24.5 | 50.3 |
| CV% | 6.0 | 2.8 | 2.7 | 9.4 | 7.7 | 8.2 |
Recovery for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
The recovery of human Shh-N spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 108 | 100-117 |
| Heparin Plasma (n=4) | 97 | 85-117 |
| Serum (n=4) | 99 | 81-117 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of human Shh-N were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
Human Sonic Hedgehog/Shh N-Terminus ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Sonic Hedgehog/Shh
Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
Alternate Names
HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS
Gene Symbol
SHH
Additional Sonic Hedgehog/Shh Products
Product Documents for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
Product Specific Notices for Human Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.