Human Serpin G1/C1 Inhibitor DuoSet ELISA

Name: Human Serpin G1/C1 Inhibitor DuoSet ELISA
Brand: R&D Systems
SKU: DY2488-05

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY2488-05

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	313 - 10,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Serpin G1/C1 Inhibitor DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human Serpin G1/C1 Inhibitor DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Serpin G1 / C1 Inhibitor. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human Serpin G1/C1 Inhibitor DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Serpin G1/C1 Inhibitor DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human Serpin G1/C1 Inhibitor DuoSet ELISA

	Human Serpin G1 / C1 Inhibitor ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin G1/C1 Inhibitor

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Item	Value
Entrez Gene IDs	710 (Human)
Alternate Names	C1 Inhibitor, C1IN, C1INH, C1NH, HAE1, HAE2, SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 , Serpin G1

Citations for Human Serpin G1/C1 Inhibitor DuoSet ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human Serpin G1/C1 Inhibitor DuoSet ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

T Casselli et al. (2017), MicroRNA and mRNA Transcriptome Profiling in Primary Human Astrocytes Infected with Borrelia burgdorferi PLoS ONE, 12(1):e0170961.
PMID: 28135303

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