Human Semaphorin 3E DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY3239-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.5-32 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Semaphorin 3E DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Semaphorin 3E DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Semaphorin 3E. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Semaphorin 3E DuoSet ELISA
Human Semaphorin 3E ELISA Standard Curve
Kit Contents for Human Semaphorin 3E DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Semaphorin 3E
Semaphorin 3E (Sema3E) is a secreted protein that functions in axon guidance and/or vascular tip cell guidance during development. Sema3E signals through Plexin D1 that may also be associated with Neuropilin 1 and/or VEGF R2. It can exert axon repulsive or attraction effects, promote axonal growth, the development of glutamatertic synaptic development, and the development of GnRH producing neurons. Sema3E also plays a role in the regulation of vascular growth and branching. It inhibits smooth muscle cell proliferation and migration in the asthmatic airway, promotes hepatic stellate cell activation and wound healing, and regulates the migration of developing thymocytes. Sema3E is upregulated by inflammatory macrophages and damaged hepatocytes. It can be cleaved to generate a form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential.
Additional Semaphorin 3E Products
Product Documents for Human Semaphorin 3E DuoSet ELISA
Product Specific Notices for Human Semaphorin 3E DuoSet ELISA
For research use only
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