Human sE-Selectin/CD62E Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DSLE00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
0.027 ng/mL
Assay Range
0.125-8 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma)
Product Summary for Human sE-Selectin/CD62E Quantikine ELISA Kit
The Quantikine Human sE-Selectin immunoassay is a 4.5 hour solid phase ELISA designed to measure sE-Selectin in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sE-Selectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sE-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human sE-Selectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human sE-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Citrate Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 1.6 | 2.9 | 4.7 | 1.5 | 2.9 | 4.6 |
| Standard Deviation | 0.11 | 0.17 | 0.24 | 0.11 | 0.25 | 0.35 |
| CV% | 6.6 | 5.7 | 5.2 | 7.3 | 8.7 | 7.7 |
Recovery for Human sE-Selectin/CD62E Quantikine ELISA Kit
The recovery of sE-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 98-110 |
| Citrate Plasma (n=4) | 96 | 85-107 |
| Heparin Plasma (n=4) | 94 | 85-107 |
| Serum (n=4) | 96 | 85-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sE-Selectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human sE-Selectin/CD62E Quantikine ELISA Kit
Human E-Selectin/CD62E ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: E-Selectin/CD62E
E-Selectin, also known as CD62E, is expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. It is a lectin that binds to sialylated or fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells. E-Selectin mediates the initial attachment of circulating leukocytes to the blood vessel wall during inflammation. This interaction permits leukocytes to roll along the vascular endothelium. Subsequent ICAM-1 and VCAM-1 mediated adhesion leads to extravasation of the leukocyte through the blood vessel wall into the extracellular matrix tissue.
Alternate Names
CD62E, ELAM1, LECAM2, SELE
Gene Symbol
SELE
Additional E-Selectin/CD62E Products
Product Documents for Human sE-Selectin/CD62E Quantikine ELISA Kit
Product Specific Notices for Human sE-Selectin/CD62E Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.