Human Osteopontin (OPN) Quantikine ELISA Kit Best Seller
Catalog # DOST00 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Length: | 4.5 hours |
Sample Type & Volume Required Per Well: | Cell Culture Supernates (50 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL) |
Sensitivity: | 0.024 ng/mL |
Assay Range: | 0.312 - 20 ng/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine, Human Milk) |
Human Osteopontin (OPN) Quantikine ELISA Kit Summary: | The Quantikine Human Osteopontin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteopontin in cell culture supernates, plasma, urine, and human milk. It contains NS0-expressed recombinant human Osteopontin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteopontin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteopontin. |
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Citations for Human Osteopontin (OPN) Quantikine ELISA Kit (69)
Citations are publications that use Bio-Techne products. Selected citations for Human Osteopontin (OPN) Quantikine ELISA Kit include:
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Species: Human
Sample Types: Urine
Downes et al. (2023-04-25), Optimizing Vancomycin Therapy in Critically Ill Children: A Population Pharmacokinetics Study to Inform Vancomycin Area under the Curve Estimation Using Novel Biomarkers Pharmaceutics
PMID: 37242578 -
Species: Human
Sample Types: Cell Culture Supernates
Pl�mers et al. (2023-04-12), Matrix Metalloproteinases Contribute to the Calcification Phenotype in Pseudoxanthoma Elasticum Biomolecules
PMID: 37189419 -
Species: Human
Sample Types: Tissue Homogenates
Y Qiu et al. (2023-02-02), Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease Proceedings of the National Academy of Sciences of the United States of America, 2023-0120(6):e2218915120.
PMID: 36730200 -
Species: Human
Sample Types: Serum
SI Karampatso et al. (2022-09-13), The Effect of a Comprehensive Life-Style Intervention Program of Diet and Exercise on Four Bone-Derived Proteins, FGF-23, Osteopontin, NGAL and Sclerostin, in Overweight or Obese Children and Adolescents Nutrients, 2022-014(18).
PMID: 36145151 -
Species: Human
Sample Types: Serum
NPE Kadoglou et al. (2022-09-01), Association of serum levels of osteopontin and osteoprotegerin with adverse outcomes after endovascular revascularisation in peripheral artery disease Cardiovascular Diabetology, 2022-021(1):171.
PMID: 36050687 -
Species: Human
Sample Types: Cell Culture Supernates
Y Lu et al. (2022-08-06), A single-cell atlas of the multicellular ecosystem of primary and metastatic hepatocellular carcinoma Nature Communications, 2022-013(1):4594.
PMID: 35933472 -
Species: Human
Sample Types: Plasma
MS Simon et al. (2022-07-21), A comprehensive approach to predicting weight gain and therapy response in psychopharmacologically treated major depressed patients: A cohort study protocol PLoS ONE, 2022-017(7):e0271793.
PMID: 35862413 -
Species: Human
Sample Types: Plasma
FM Trovato et al. (2022-06-24), Immunometabolic analysis shows a distinct cyto-metabotype in Covid-19 compared to sepsis from other causes Heliyon, 2022-08(6):e09733.
PMID: 35774516 -
Species: Human
Sample Types: Cell Culture Supernates
JJ Wu et al. (2022-06-06), The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis Cell Death Disease, 2022-06-06;13(6):527.
PMID: 35661105 -
Species: Human
Sample Types: Serum
Y Cheng et al. (2022-04-06), Genetics of osteopontin in patients with chronic kidney disease: The German chronic kidney disease study PloS Genetics, 2022-018(4):e1010139.
PMID: 35385482 -
Species: Human
Sample Types: Urine
B Moszczuk et al. (2022-03-22), Osteopontin-A Potential Biomarker for IgA Nephropathy: Machine Learning Application Biomedicines, 2022-010(4).
PMID: 35453484 -
Species: Human
Sample Types: Cell Culture Supernates
JY Lim et al. (2022-03-18), Protective Effect of Human-Neural-Crest-Derived Nasal Turbinate Stem Cells against Amyloid-beta Neurotoxicity through Inhibition of Osteopontin in a Human Cerebral Organoid Model of Alzheimer's Disease Cells, 2022-011(6).
PMID: 35326480 -
Species: Human
Sample Types: Plasma
A Rühle et al. (2022-02-21), The value of plasma hypoxia markers for predicting imaging-based hypoxia in patients with head-and-neck cancers undergoing definitive chemoradiation Clinical and translational radiation oncology, 2022-033(0):120-127.
PMID: 35243023 -
Species: Human
Sample Types: Plasma
SE Mortazavi et al. (2021-11-06), Osteopontin and malaria: no direct effect on parasite growth, but correlation with P. falciparum-specific B cells and BAFF in a malaria endemic area BMC microbiology, 2021-121(1):307.
PMID: 34742229 -
Species: Human
Sample Types: Cell Culture Supernates
X Zhang et al. (2021-10-19), Interleukin-33 as an early predictor of cetuximab treatment efficacy in patients with colorectal cancer Cancer Medicine, 2021-10(0).
PMID: 34664425 -
Species: Human
Sample Types: Breast Milk
A Aksan et al. (2021-07-31), Osteopontin Levels in Human Milk Are Related to Maternal Nutrition and Infant Health and Growth Nutrients, 2021-013(8).
PMID: 34444830 -
Species: Human
Sample Types: Plasma
Z Lin et al. (2021-05-18), Evaluation of a Three-Marker Panel for the Detection of Uveal Melanoma Metastases: A Single-Center Retrospective Analysis Cancers, 2021-013(10).
PMID: 34070192 -
Species: Human
Sample Types: Cell Culture Supernates
T Seki et al. (2021-04-08), Interleukin-6 as an enhancer of anti-angiogenic therapy for ovarian clear cell carcinoma Scientific Reports, 2021-011(1):7689.
PMID: 33833265 -
Species: Human, Mouse
Sample Types: Cell Culture Supernates, Serum
X Jiang et al. (2021-02-27), Lipid-injured hepatocytes release sOPN to improve macrophage migration via CD44 engagement and pFak-NF&kappaB signaling Cytokine, 2021-0142(0):155474.
PMID: 33647584 -
Species: Human
Sample Types: Plasma
YL Chai et al. (2021-02-17), Plasma osteopontin as a biomarker of Alzheimer's disease and vascular cognitive impairment Scientific Reports, 2021-011(1):4010.
PMID: 33597603 -
Species: Human
Sample Types: Cell Culture Supernates
K Tokuda et al. (2021-02-16), The interaction between cancer associated fibroblasts and tumor associated macrophages via the osteopontin pathway in the tumor microenvironment of hepatocellular carcinoma Oncotarget, 2021-012(4):333-343.
PMID: 33659044 -
Species: Human
Sample Types: Plasma
S Frankova et al. (2021-01-07), Liver stiffness measured by two-dimensional shear-wave elastography predicts hepatic vein pressure gradient at high values in liver transplant candidates with advanced liver cirrhosis PLoS ONE, 2021-016(1):e0244934.
PMID: 33411729 -
Species: Human
Sample Types: Plasma
T Takeuchi et al. (2020-10-21), ASP5094, a humanized monoclonal antibody against integrin alpha-9, did not show efficacy in patients with rheumatoid arthritis refractory to methotrexate: results from a phase 2a, randomized, double-blind, placebo-controlled trial Arthritis Res Ther, 2020-122(1):252.
PMID: 33087159 -
Species: Human
Sample Types: Serum
C Xu et al. (2020-09-01), Prognostic significance of serum osteopontin levels in small cell lung cancer BMC Pulm Med, 2020-020(1):235.
PMID: 32873264 -
Species: Human
Sample Types: Cell Culture Supernates
B Gómez-Sant et al. (2020-07-07), Liver osteopontin is required to prevent the progression of age-related nonalcoholic fatty liver disease Aging Cell, 2020-00(0):e13183.
PMID: 32638492 -
Species: Human
Sample Types: Plasma
C Wang et al. (2019-09-09), Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus Cytokine, 2019-0125(0):154837.
PMID: 31514105 -
Species: Human
Sample Types: Plasma
R Suwinski et al. (2019-05-08), Blood serum proteins as biomarkers for prediction of survival, locoregional control and distant metastasis rate in radiotherapy and radio-chemotherapy for non-small cell lung cancer BMC Cancer, 2019-019(1):427.
PMID: 31068179 -
Species: Human
Sample Types: Cell Culture Supernates
SH Choi et al. (2018-11-30), Tumour-vasculature development via endothelial-to-mesenchymal transition after radiotherapy controls CD44v6+ cancer cell and macrophage polarization Nat Commun, 2018-19(1):5108.
PMID: 30504836 -
Species: Human
Sample Types: Plasma
CK Wu et al. (2018-11-08), Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients J Clin Med, 2018-17(11).
PMID: 30413105 -
Species: Human
Sample Types: Serum
JD Klement et al. (2018-11-05), An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion J. Clin. Invest., 2018-10(0).
PMID: 30395540 -
Species: Human
Sample Types: Urine
K Jobs et al. (2018-09-26), Assessment of Cross-correlations Between Selected Macromolecules in Urine of Children with Idiopathic Hypercalciuria Urol J, 2018-00(0).
PMID: 29353465 -
Species: Human
Sample Types: Cell Culture Supernates
T Shimodaira et al. (2018-04-25), Upregulation of osteopontin expression via the interaction of macrophages and fibroblasts under IL-1b stimulation Cytokine, 2018-0110(0):63-69.
PMID: 29704820 -
Species: Human
Sample Types: Cell Culture Supernates
X Jin et al. (2018-03-14), Methylglyoxal-bis-guanylhydrazone inhibits osteopontin expression and differentiation in cultured human monocytes PLoS ONE, 2018-013(3):e0192680.
PMID: 29538412 -
Species: Human
Sample Types: Plasma
X Zhang et al. (2018-02-15), Association of plasma osteopontin with diabetic retinopathy in Asians with type 2 diabetes Mol. Vis., 2018-024(0):165-173.
PMID: 29463954 -
Species: Human
Sample Types: Whole Blood
M Giakoumis et al. (2018-01-04), Effects of oral paricalcitol therapy on arterial stiffness and osteopontin in hypertensive patients with chronic kidney disease and secondary hyperparathyroidism Hellenic J Cardiol, 2018-00(0).
PMID: 29305902 -
Species: Human
Sample Types: Cell Culture Supernates
N Gan et al. (2017-05-12), Osteopontin is Critical for Hyperactive mTOR-Induced Tumorigenesis in Oral Squamous Cell Carcinoma J Cancer, 2017-08(8):1362-1370.
PMID: 28638450 -
Species: Human
Sample Types: Cell Culture Supernates
(2016-12-09), Epigallocatechin-3-Gallate Upregulates miR-221 to Inhibit Osteopontin-Dependent Hepatic Fibrosis PLoS ONE, 2016-111(12):e0167435.
PMID: 27935974 -
Species: Human
Sample Types: Cell Culture Supernates
(2016-12-08), Development of an in vitro human liver system for interrogating nonalcoholic steatohepatitis JCI Insight, 2016-11(20):e90954.
PMID: 27942596 -
Species: Human
Sample Types: Cell Culture Supernates
(2016-11-15), IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 2016-190(0):88-95.
PMID: 27863335 -
Species: Human
Sample Types: Cell Culture Supernates
(2016-08-24), Targeting of the leukemia microenvironment by c(RGDfV) overcomes the resistance to chemotherapy in acute myeloid leukemia in biomimetic polystyrene scaffolds Oncol Lett, 2016-012(5):3278-3284.
PMID: 27899994 -
Species: Human
Sample Types: Plasma
(2016-07-19), Immunomodulation Induced by Stem Cell Mobilization and Harvesting in Healthy Donors: Increased Systemic Osteopontin Levels after Treatment with Granulocyte Colony-Stimulating Factor Int J Mol Sci, 2016-017(7).
PMID: 27447610 -
Species: Human
Sample Types: Cell Culture Supernates
(2016-05-31), Osteopontin is a multi-faceted pro-tumorigenic driver for central nervous system lymphoma Oncotarget, 2016-07(22):32156-71.
PMID: 27050077 -
Species: Human
Sample Types: Serum
Stilund M et al. (2015-04-10), Biomarkers of inflammation and axonal degeneration/damage in patients with newly diagnosed multiple sclerosis: contributions of the soluble CD163 CSF/serum ratio to a biomarker panel. PLoS ONE, 2015-010(4):e0119681.
PMID: 25860354 -
Species: Human
Sample Types: Plasma
Arsenault B et al. (2014-12-22), Prediction of cardiovascular events in statin-treated stable coronary patients of the treating to new targets randomized controlled trial by lipid and non-lipid biomarkers. PLoS ONE, 2014-19(12):e114519.
PMID: 25531109 -
Species: Human
Sample Types: Serum
Aderhold C et al. (2014-07-04), Interleukin 4, interleukin 6 and osteopontin-serological markers of head and neck malignancy in primary diagnostics: A pilot study. Oncol Lett, 2014-08(3):1112-1118.
PMID: 25120668 -
Species: Human
Sample Types: Pleural Fluid
Zhang et al. (2014-04-23), Prognostic value of secreted phosphoprotein-1 in pleural effusion associated with non-small cell lung cancer. BMC Cancer, 2014-014(0):280.
PMID: 24758329 -
Species: Human
Sample Types: Serum
Rud A et al. (2013-11-11), Osteopontin is a prognostic biomarker in non-small cell lung cancer. BMC Cancer, 2013-113(0):540.
PMID: 24215488 -
Species: Human
Sample Types: Tissue Homogenates
Yamaguchi Y et al. (2012-11-30), Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage. J Biol Chem, 2012-1288(5):3097-111.
PMID: 23204518 -
Species: Human
Sample Types: Urine
Goodison et al. (2012-10-19), A multi-analyte assay for the non-invasive detection of bladder cancer. PLoS ONE, 2012-17(10):e47469.
PMID: 23094052 -
Species: Human
Sample Types: Plasma
(2012-08-23), Prognostic utility of pre-operative circulating osteopontin, carbonic anhydrase IX and CRP in renal cell carcinoma. Br. J. Cancer, 2012-0107(7):1131-7.
PMID: 22918393 -
Species: Human
Sample Types: Plasma
Liersch R et al. (2012-04-17), Osteopontin is a prognostic factor for survival of acute myeloid leukemia patients. Blood, 2012-0119(22):5215-20.
PMID: 22510874 -
Species: Human
Sample Types: Plasma
Servais EL et al. (2011-10-26), An in vivo platform for tumor biomarker assessment. PLoS ONE, 2011-16(10):e26722.
PMID: 22046338 -
Species: Human
Sample Types: Serum
Zekri A et al. (2011-06-21), Serum levels of beta-catenin as a potential marker for genotype 4/hepatitis C-associated hepatocellular carcinoma. Oncol Rep, 2011-026(4):825-31.
PMID: 21701780 -
Species: Human
Sample Types: Plasma
Kluger HM et al. (2011-04-12), Plasma markers for identifying patients with metastatic melanoma. Clin. Cancer Res., 2011-017(8):2417-25.
PMID: 21487066 -
Species: Human
Sample Types: Serum
Farlow EC et al. (2010-09-21), A multi-analyte serum test for the detection of non-small cell lung cancer. Br. J. Cancer, 2010-0103(8):1221-8.
PMID: 20859284 -
Species: Human
Sample Types: Plasma
Grimm G et al. (2010-04-24), Changes in osteopontin and in biomarkers of bone turnover during human endotoxemia. Bone, 2010-047(2):388-91.
PMID: 20420943 -
Species: Human
Sample Types: Plasma
Lorenzen J et al. (2010-04-01), Circulating levels of osteopontin are closely related to glomerular filtration rate and cardiovascular risk markers in patients with chronic kidney disease. Eur. J. Clin. Invest., 2010-040(4):294-300.
PMID: 20486990 -
Species: Human
Sample Types: Plasma
Georgiadou P et al. (2010-04-01), Osteopontin as a novel prognostic marker in stable ischaemic heart disease: a 3-year follow-up study. Eur. J. Clin. Invest., 2010-00(0).
PMID: 20192976 -
Species: Human
Sample Types: BALF
Puxeddu I et al. (2009-10-05), Osteopontin is expressed and functional in human eosinophils. Allergy, 2009-165(2):168-74.
PMID: 19804447 -
Species: Human
Sample Types: Cell Culture Supernates
Campeau PM et al. (2009-07-08), Characterization of Gaucher disease bone marrow mesenchymal stromal cells reveals an altered inflammatory secretome. Blood, 2009-0114(15):3181-90.
PMID: 19587377 -
Species: Human
Sample Types: Plasma
Castellano G et al. (2008-11-15), Activation of the osteopontin/matrix metalloproteinase-9 pathway correlates with prostate cancer progression. Clin. Cancer Res., 2008-114(22):7470-80.
PMID: 19010864 -
Species: Human
Sample Types: Plasma
Corvol JC et al. (2008-08-08), Abrogation of T cell quiescence characterizes patients at high risk for multiple sclerosis after the initial neurological event. Proc. Natl. Acad. Sci. U.S.A., 2008-0105(33):11839-44.
PMID: 18689680 -
Species: Human
Sample Types: Cell Culture Supernates
Zdzisinska B et al. (2008-05-30), Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells. Arch. Immunol. Ther. Exp. (Warsz.), 2008-056(3):207-21.
PMID: 18512025 -
Species: Human
Sample Types: Plasma
Kadoglou NP et al. (2008-04-18), Intensive lipid-lowering therapy ameliorates novel calcification markers and GSM score in patients with carotid stenosis. Eur J Vasc Endovasc Surg, 2008-035(6):661-8.
PMID: 18395477 -
Species: Human
Sample Types: Cell Culture Supernates
Dower K et al. (2008-03-01), Innate immune responses to TREM-1 activation: overlap, divergence, and positive and negative cross-talk with bacterial lipopolysaccharide. J. Immunol., 2008-0180(5):3520-34.
PMID: 18292579 -
Species: Human
Sample Types: Cell Culture Supernates
Colla S et al. (2007-09-11), The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis. Blood, 2007-0110(13):4464-75.
PMID: 17848618 -
Species: Human
Sample Types: Cell Culture Supernates
Shao J et al. (2007-08-11), Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin. Carcinogenesis, 2007-028(12):2476-83.
PMID: 17693663 -
Species: Human
Sample Types: Serum
Meinhold-Heerlein I et al. (2007-01-15), An integrated clinical-genomics approach identifies a candidate multi-analyte blood test for serous ovarian carcinoma. Clin. Cancer Res., 2007-013(2):458-66.
PMID: 17255266 -
Species: Human
Sample Types: Serum
Pe'er J (2006-03-01), Osteopontin expression and serum levels in metastatic uveal melanoma: a pilot study. Invest. Ophthalmol. Vis. Sci., 2006-047(3):802-6.
PMID: 16505010
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Customer Reviews for Human Osteopontin (OPN) Quantikine ELISA Kit (2)
Customer Images
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Sample Tested: SerumVerified Customer | Posted 02/18/2021
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Sample Tested: Human cell conditioned mediumVerified Customer | Posted 12/01/2017
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Product Documents for Human Osteopontin (OPN) Quantikine ELISA Kit
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.