Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

Name: Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA
Brand: R&D Systems
SKU: DYC3419-2

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DYC3419-2

Citations (19)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	312.5 - 20,000 pg/mL
Sample Types:	Cell Lysates
Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Available in 2, 5, and 15- (96-well) plate pack sizes Economical alternative to Western blot

View Full Technical Details for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

Summary for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure total SOD2 / Mn-SOD in cell lysates. An immobilized capture antibody specific for SOD2 / Mn-SOD binds both phosphorylated and unphosphorylated SOD2 / Mn-SOD. After washing away unbound material, a biotinylated detection antibody is used to detect both phosphorylated and unphosphorylated protein, utilizing a standard Streptavidin-HRP format.

Product Specifications for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	Cell lysates (100 µL)
Reactivity	Human, Mouse, Rat
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	Kits available for two, five, or fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data Examples for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

	Standard Curve
	Detection of SOD2/Mn-SOD in HepG2 Cell Line Lysate. Lysates prepared from HepG2 human hepatocellular carcinoma cells were incubated in wells coated with Human/Mouse/Rat Total SOD2/Mn-SOD Capture Antibody. Unbound material was removed by washing and bound material was solubilized in SDS gel sample buffer. The same lysate and captured protein were electrophoresed, transferred to a PVDF membrane, and immunoblotted with a Human/Mouse/Rat SOD2 Polyclonal Antibody (R&D Systems®, Catalog # AF3419). Only the band corresponding to SOD2 was detected in captured material. To further determine specificity, recombinant human SOD1 and recombinant human SOD3 were assayed at 200 ng/mL and did not cross-react or interfere in the assay.
	Detection of SOD2/Mn-SOD in Cancer Cell Lysate. Lysates prepared from HepG2 human hepatocellular carcinoma cells, A172 human glioblastoma cells, HeLa human cervical epithelial carcinoma cells, Jurkat human acute T cell leukemia cells, and MDA-MB-453 human breast cancer cells were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of human/mouse/rat SOD2 detected by Western Blot.
	Detection of SOD2/Mn-SOD in Human PBMC Lysate. Human peripheral blood mononuclear cells (PBMCs) were cultured for 24 hours in the absence or presence of 1 mM lipopolysaccharide (LPS). The lysates were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of human/mouse/rat SOD2 detected by Western Blot. Amounts of SOD2 as quantified by the Human/Mouse/Rat Total SOD2/Mn-SOD DuoSet® IC ELISA are consistent with the relative amounts of SOD2 determined by qualitative Western blot analysis.
	Detection of SOD2/Mn-SOD in Cell Lysate. Lysates prepared from DA3 mouse myeloma cells, L-929 mouse fibroblast cells, and PC-12 rat adrenal pheochromocytoma cells were quantified with this DuoSet® IC ELISA. The same lysates were immunoblotted (inset) with an anti-SOD2 polyclonal antibody. The DuoSet® IC ELISA results correlate well with the relative amounts of SOD2 detected by Western Blot.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SOD2/Mn-SOD

Superoxide Dismutases, originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Three mammalian isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SODs are typically soluble secreted or cytosolic proteins, but are also found in the mitochondria and extracellular matrix.

Any of three metals, manganese, iron, or copper, may be used in the active site of SOD and are indicative of cellular localization.MnSOD (SOD2) is found in the mitochondria of both prokaryotes and ukaryotes, whereas the cytosol of eukaryotes and prokaryotic organisms contains Cu/ZnSOD (SOD1) and FeSOD, respectively.

There have been several mutations identified in the Cu/ZnSOD (SOD1) gene in amyotrophic lateral sclerosis (ALS) patients, possibly suggesting a role for free radicals in this disease process. The mechanism of motor neuron degeneration that occurs in ALS, however, is unclear at this time. Several hypotheses have been explored for the role of mutant SOD1 and convincing evidence for the involvement of apoptosis has been presented, as well.

Item	Value
Long Name	Superoxide Dismutase-2
Entrez Gene IDs	6648 (Human); 20656 (Mouse); 24787 (Rat)
Alternate Names	EC 1.15.1.1, IPO-B, IPOB, MVCD6, Mn SOD, Mn superoxide dismutase, MnSOD, SOD, Mitochodrial, SOD2, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial, superoxide dismutase [Mn], mitochondrial

Citations for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA (19)

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA include:

Species: Mouse
Sample Types: Cell Culture Supernatants

R D'Amico et al. (2022), Toxic Effects of Endocrine Disruptor Exposure on Collagen-Induced Arthritis Biomolecules, 12(4).
PMID: 35454153
Species: Rat
Sample Types: Serum

LQ Chen et al. (2022), Minocycline relieves myocardial ischemia-reperfusion injury in rats by inhibiting inflammation, oxidative stress and apoptosis European review for medical and pharmacological sciences, 26(8):3001-3009.
PMID: 35503600
Species: Rat
Sample Types: Tissue Homogenates

IA Alsharif et al. (2022), Miconazole Mitigates Acetic Acid-Induced Experimental Colitis in Rats: Insight into Inflammation, Oxidative Stress and Keap1/Nrf-2 Signaling Crosstalk Biology, 11(2).
PMID: 35205169
Species: Human
Sample Types: Cell Lysates

OM David et al. (2022), The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO2NPs) Using Caco-2 Cells Biomolecules, 12(10).
PMID: 36291543
Species: Rat
Sample Types: Tissue Homogenates

M Mohany et al. (2022), The Role of NF-kappaB and Bax/Bcl-2/Caspase-3 Signaling Pathways in the Protective Effects of Sacubitril/Valsartan (Entresto) against HFD/STZ-Induced Diabetic Kidney Disease Biomedicines, 10(11).
PMID: 36359384
Species: Human
Sample Types: Cell Lysates

CH Tsai et al. (2021), Aldosterone Excess Induced Mitochondria Decrease and Dysfunction via Mineralocorticoid Receptor and Oxidative Stress In Vitro and In Vivo Biomedicines, 9(8).
PMID: 34440149
Species: Mouse
Sample Types: Tissue Homogenates

X Wang et al. (2021), AKR1C1 alleviates LPS?induced ALI in mice by activating the JAK2/STAT3 signaling pathway Molecular Medicine Reports, 24(6).
PMID: 34590152
Species: Rat
Sample Types: Plasma

R Wang et al. (2021), Mangiferin exert protective effects on joints of adjuvant-induced arthritis rats by regulating the MAPKs/NF-kappaB pathway of fibroblast-like synoviocytes International immunopharmacology, 0(0):108352.
PMID: 34836794
Species: Rat
Sample Types: Tissue Homogenates

HM Abuohashis et al. (2021), Pharmacological Effects of Marine-Derived Enterococcus faecium EA9 against Acute Lung Injury and Inflammation in Cecal Ligated and Punctured Septic Rats BioMed Research International, 2021(0):5801700.
PMID: 34912891
Species: Mouse
Sample Types: Serum

LR Ling et al. (2020), Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes Oncol Lett, 19(4):2861-2869.
PMID: 32218840
Species: Human
Sample Types: Cell Lysates

G Haji et al. (2020), Mitochondrial dysfunction in airways and quadriceps muscle of patients with chronic obstructive pulmonary disease Respir Res, 21(1):262.
PMID: 33046036
Species: Rat
Sample Types: Tissue Homogenates

M Mohany et al. (2020), LCZ696 mitigates diabetic-induced nephropathy through inhibiting oxidative stress, NF-kappaB mediated inflammation and glomerulosclerosis in rats PeerJ, 8(0):e9196.
PMID: 32596035
Species: Rat
Sample Types: Tissue Homogenates

SQ Rodríguez- et al. (2019), Effect of Telmisartan in the Oxidative Stress Components Induced by Ischemia Reperfusion in Rats Oxid Med Cell Longev, 2019(0):1302985.
PMID: 31354899
Species: Mouse
Sample Types: Tissue Homogenates

R Tan et al. (2019), Effect of transcutaneous electrical acupoint stimulation on protecting against radiotherapy- induced ovarian damage in mice J Ovarian Res, 12(1):65.
PMID: 31324205
Species: Rat
Sample Types: Cell Culture Supernates

X Meng et al. (2018), Protective effect and mechanism of rat recombinant S100 calcium-binding protein A4 on oxidative stress injury of rat vascular endothelial cells Oncol Lett, 16(3):3614-3622.
PMID: 30127969
Species: Rat
Sample Types: Tissue Homogenates

CH Chuang et al. (2018), Acute renal injury induced by endotoxic shock in rats is alleviated via PI3K/Nrf2 pathway Eur Rev Med Pharmacol Sci, 22(16):5394-5401.
PMID: 30178867
Species: Mouse
Sample Types: Tissue Homogenates

XN Wang et al. (2017), Protective Effects of Curcumin against Sodium Arsenite-induced Ovarian Oxidative Injury in a Mouse Model Chin. Med. J., 130(9):1026-1032.
PMID: 28469096
Species: Nematode
Sample Types: Cell Culture Supernates

(2016), Multigenerational Effects of Heavy Metals on Feeding, Growth, Initial Reproduction and Antioxidants in Caenorhabditis elegans PLoS ONE, 11(4):e0154529.
PMID: 27116222
Species: Rat
Sample Types: Tissue Homogenates

Mao S et al. (2016), Gastroprotective Effects of Astragaloside IV against Acute Gastric Lesion in Rats. PLoS ONE, 11(2):e0148146.
PMID: 26845156

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