Human/Mouse/Rat SOD2/Mn-SOD Antibody

Detection of Human/Mouse/Rat SOD2/Mn-SOD by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOD2/Mn-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOD2/Mn-SOD at approximately 23 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Detection of Human and Mouse SOD2/Mn‑SOD by Simple Western^TM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD2/Mn-SOD at approximately 28 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOD2/Mn-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse SOD2/Mn-SOD by Western Blot

CD138low cells have altered mitochondrial properties that can be utilized to induce oxidative stress by 10-TPP treatment E. HMCLs transduced with Ad-CMV or Ad-MnSOD, treated with 10-TPP,&MitoSOX oxidation was analyzed by flow cytometry. Data is presented as fold change normalized to control cells expressing Ad-CMV. Antimycin A treatment was used as a positive control. *p < 0.01 vs. control cells (Ad-CMV or Ad-MnSOD), #p < 0.05 vs. 10-TPP treated Ad-CMV cells. Representative WB of HMCLs transduced with Ad-CMV or Ad-MnSOD (MOI = 50), whole-cell extract was made at 48 h&probed with an antibody against MnSOD (24 kDa) or beta-actin. The quantification of MnSOD after normalization to untreated control is shown below each band. F. Structure of 10-TPVP; representative confocal images of MM.1S&OPM-2 cells stained with MitoTracker red&10-TPVP. The bright field view&merge image of MitoTracker Red&10-TPVP are also shown. For panels B-E, bars represent mean of three independent runs ± SEM. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27902770), licensed under a CC-BY license. Not internally tested by R&D Systems.