Human MMP-2 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY902
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.625-20 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human MMP-2 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human MMP-2 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MMP-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human MMP-2 DuoSet ELISA
Human MMP-2 ELISA Standard Curve
Kit Contents for Human MMP-2 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-2
MMP-2 (matrix metalloproteinase 2), also known as Gelatinase A, is upregulated at sites of tissue damage, inflammation, and in stromal cells surrounding the invading front of metastatic tumors. The latent proenzyme (proMMP-2) is activated by MT-MMPs, Thrombin, or activated Protein C. Mature MMP-2 activity is inhibited by alpha 2-Macroglobulin or TIMPs 1, 2, 3, and -4. TIMP-2 and -3 can also tether proMMP-2 into cell surface ternary complexes with MT-MMPs. MMP-2 degrades Gelatin (denatured Collagen) and type IV Collagen, the major component of basement membranes. It can also degrade the matrix proteins Collagens V, VII and X, Decorin, Elastin, and Fibronectin. MMP-2 processes and modulates the functions of many other vasoactive and proinflammatory molecules including Adrenomedullin, Big Endothelin-1, Calcitonin gene-related peptide, CCL7/MCP-3, CXCL12/SDF-1, Galectin-3, IGFBP-3, IL-1 beta, S100A8, and S100A9.
Long Name
Matrix Metalloproteinase 2
Alternate Names
Gelatinase A, MMP2
Gene Symbol
MMP2
Additional MMP-2 Products
Product Documents for Human MMP-2 DuoSet ELISA
Product Specific Notices for Human MMP-2 DuoSet ELISA
For research use only
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