Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Human MIF DuoSet ELISA

Bio-Techne includes R&D Systems | Catalog # DY289

Catalog #
Availability
Size / Price
Qty
Loading...
DY289

Ancillary Products Available

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

31.2-2000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human MIF DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Product Summary for Human MIF DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MIF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Label

HRP

Scientific Data Images for Human MIF DuoSet ELISA

Human MIF ELISA Standard Curve

Human MIF ELISA Standard Curve

Kit Contents for Human MIF DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MIF

MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.

Long Name

Macrophage Migration Inhibitory Factor

Alternate Names

EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase

Entrez Gene IDs

4282 (Human); 17319 (Mouse)

Gene Symbol

MIF

Additional MIF Products

Product Documents for Human MIF DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human MIF DuoSet ELISA

For research use only

Citations for Human MIF DuoSet ELISA (32)

Showing  1 - 10 of 32 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human MIF DuoSet ELISA include:

There are no citations that match your criteria.

Showing  1 - 10 of 32 publications Showing All

Customer Reviews for Human MIF DuoSet ELISA (3)

5 out of 5
3 customer ratings
5 stars
100%
4 stars
0%
3 stars
0%
2 stars
0%
1 star
0%

Customer Images


Showing  1 - 3 of 3 reviews Showing All
Filter By:
  • Human MIF DuoSet ELISA
    Name: Anonymous
    Sample Tested: iDC human immature myeloid dendritic cells
    Verified Customer | Posted 11/01/2022
    We used two batch lot number of this kit. Both work good.
    Human MIF DuoSet ELISA DY289
  • Human MIF DuoSet ELISA
    Name: Anonymous
    Sample Tested: iDC human immature myeloid dendritic cells
    Verified Customer | Posted 10/04/2022
    Human MIF DuoSet ELISA DY289
  • Human MIF DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 07/06/2021
    we used this kit to quantify human MIF in serum and plasma samples. Works very well and easy to use protocol.
    Human MIF DuoSet ELISA DY289

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human MIF DuoSet ELISA

Showing  1 - 1 of 1 FAQ Showing All
  • Q: Sera appears to have ng levels of MIF present. Since homology between bovine and human is 92%, will media supplemented with FCS present any problems.

    A: Bovine MIF has not been evaluated, so running a media control would be recommended.

Showing  1 - 1 of 1 FAQ Showing All