Human Mesothelin Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DMSLN0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (50 µL)
Sensitivity
0.022 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Product Summary for Human Mesothelin Quantikine ELISA Kit
The Quantikine Human Mesothelin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Mesothelin in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human Mesothelin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Mesothelin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Mesothelin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Mesothelin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.83 | 2.56 | 5.54 | 0.91 | 2.80 | 5.94 |
| Standard Deviation | 0.034 | 0.082 | 0.193 | 0.057 | 0.130 | 0.360 |
| CV% | 4.0 | 3.2 | 3.5 | 6.2 | 4.6 | 6.1 |
Recovery for Human Mesothelin Quantikine ELISA Kit
The recovery of Mesothelin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 104 | 91-113 |
| EDTA Plasma (n=4) | 97 | 88-109 |
| Heparin Plasma (n=4) | 97 | 86-112 |
| Saliva (n=4) | 102 | 91-112 |
| Serum (n=4) | 99 | 92-114 |
| Urine (n=4) | 103 | 86-115 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of Mesothelin were serially diluted Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Mesothelin Quantikine ELISA Kit
Human Mesothelin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Mesothelin
Mesothelin, also known as CAK1 and ERC, is derived from a precursor that also includes Megakaryocyte Potentiating Factor (MPF). Following cleavage of the precursor, Mesothelin remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.
Alternate Names
CAK1, MPF, MSLN, SMR
Gene Symbol
MSLN
Additional Mesothelin Products
Product Documents for Human Mesothelin Quantikine ELISA Kit
Product Specific Notices for Human Mesothelin Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.