Human Lysyl Oxidase Homolog 2 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY2639-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.375-24 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Lysyl Oxidase Homolog 2 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Lysyl Oxidase Homolog 2 DuoSet ELISA
Human Lysyl Oxidase Homolog 2 / LOXL2 ELISA Standard Curve
Kit Contents for Human Lysyl Oxidase Homolog 2 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Lysyl Oxidase Homolog 2/LOXL2
Lysyl Oxidase Homolog 2 is one of several secreted copper-binding amine oxidases that oxidize primary amine substrates to aldehydes. It is widely expressed, particularly in placenta, uterus, and prostate. LOXL2 is upregulated during cancer progression and promotes cell migration and tumor cell invasiveness.
Alternate Names
LOL2, LOXL2
Gene Symbol
LOXL2
Additional Lysyl Oxidase Homolog 2/LOXL2 Products
Product Documents for Human Lysyl Oxidase Homolog 2 DuoSet ELISA
Product Specific Notices for Human Lysyl Oxidase Homolog 2 DuoSet ELISA
For research use only
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