Human LIGHT/TNFSF14 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DLIT00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (25 µL)
Sensitivity
16.5 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Product Summary for Human LIGHT/TNFSF14 Quantikine ELISA Kit
The Quantikine Human LIGHT Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human LIGHT in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human LIGHT and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human LIGHT showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human LIGHT.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human LIGHT
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 308 | 626 | 1181 | 318 | 616 | 1177 |
| Standard Deviation | 11.7 | 25.3 | 34.6 | 27.0 | 44.2 | 81.8 |
| CV% | 3.8 | 4.0 | 2.9 | 8.5 | 7.2 | 6.9 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 350 | 712 | 1388 | 387 | 754 | 1469 |
| Standard Deviation | 12.0 | 31.7 | 63.3 | 19.6 | 31.8 | 63.4 |
| CV% | 3.4 | 4.5 | 4.6 | 5.1 | 4.2 | 4.3 |
Recovery for Human LIGHT/TNFSF14 Quantikine ELISA Kit
The recovery of LIGHT spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 100-113 |
| EDTA Plasma (n=5) | 106 | 91-114 |
| Heparin Plasma (n=5) | 101 | 89-115 |
| Serum (n=5) | 102 | 85-114 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of LIGHT were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human LIGHT/TNFSF14 Quantikine ELISA Kit
Human LIGHT/TNFSF14 ELISA Calibrator Diluent RD5K Standard Curve
Human LIGHT/TNFSF14 ELISA Calibrator Diluent RD6-6 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LIGHT/TNFSF14
LIGHT, also known as TNFSF14, is a homotrimeric glycoprotein in the TNF superfamily. It is expressed on the surface of activated lymphocytes, NK cells, immature dendritic cells, monocytes, and granulocytes. A soluble form can be shed by proteolysis. LIGHT binds and activates HVEM/TNFRSF14 to costimulate Th1 immune responses, enhance CD8+ T cell‑mediated tumor immunity, and regulate allogeneic T cell activation and allograft rejection. In addition, it binds and activates Lymphotoxin beta R/TNFRSF3 to induce apoptosis, cytokine production, mesenteric lymph node organogenesis, and maintenance of lymph node structure and function. It can also bind the soluble human decoy receptor DcR3/TNFRSF6B.
Long Name
TNF Ligand Superfamily Member 14
Alternate Names
CD258, TNFSF14
Gene Symbol
TNFSF14
Additional LIGHT/TNFSF14 Products
Product Documents for Human LIGHT/TNFSF14 Quantikine ELISA Kit
Product Specific Notices for Human LIGHT/TNFSF14 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.