Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY6030B-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
0.469-30 ng/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Indoleamine 2,3-dioxygenase (IDO). The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
Human IDO ELISA Standard Curve
Kit Contents for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (R&D Systems,Catalog # DY999B)
Stop Solution:2N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990), or equivalent
Plate Sealers:ELISA Plate Sealers (Catalog #DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Indoleamine 2,3-dioxygenase/IDO
Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN gamma, IFN alpha, IFN beta, and IL-10. IDO has an antimicrobial function and induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia.
Alternate Names
3dioxygenase, IDO, IDO1, INDO, Indoleamine 2
Gene Symbol
IDO1
Additional Indoleamine 2,3-dioxygenase/IDO Products
Product Documents for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
Product Specific Notices for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
For research use only
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