Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Name: Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA
Brand: R&D Systems
SKU: DY6030-05
Rating: 3 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY6030-05

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Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	0.469 - 30 ng/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Summary for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Indoleamine 2,3-dioxygenase (IDO). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

	Human Indoleamine 2,3-dioxygenase / IDO ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN gamma, IFN alpha, IFN beta, and IL-10. IDO has an antimicrobial function and induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia.

Item	Value
Entrez Gene IDs	3620 (Human); 15930 (Mouse)
Alternate Names	3dioxygenase, EC 1.13.11.52, IDO, IDO1, IDOIDO-1, INDO, INDOindole 2,3-dioxygenase, Indoleamine 2, Indoleamine 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase, indoleamine 2,3-dioxygenase 1, indoleamine-pyrrole 2,3 dioxygenase

Citations for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

S Nürnberger et al. (2019), Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction Eur Cell Mater, 37(0):113-133.
PMID: 30793275

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Customer Reviews for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA (1)

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Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Name: shelley Cutrone

Sample Tested: Cell culture supernatant

Verified Customer | Posted 11/05/2018

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Product Documents for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

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Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

R&D Systems, Inc. a Bio-Techne Brand

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Summary for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Product Specifications for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Kit Contents for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Preparation and Storage

Background: Indoleamine 2,3-dioxygenase/IDO

Citations for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA (1)

Customer Reviews for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA (1)

Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Product Documents for Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA

Product Datasheets

COA

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SDS

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