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Human IL-4 Quantikine ELISA Kit

Human IL-4 ELISA Cell Culture Supernate Standard Curve
(3)
Human IL-4 ELISA Serum/Plasma Standard Curve
Human IL-4 ELISA Linearity
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PD4050
S4050
D4050

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Key Product Details

Assay Length: 4.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
Sample Types: Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Sensitivity: 10 pg/mL
Assay Range: 31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Human IL-4 Quantikine ELISA Kit Summary: The Quantikine Human IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-4 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human IL-4 accurately. Results obtained using natural human IL-4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-4.

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Citations for Human IL-4 Quantikine ELISA Kit (59)

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Customer Reviews (2)

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  • Human IL-4 Quantikine ELISA Kit
    Name: Alisha Freeman
    Sample Tested: 293T human embryonic kidney cell line
    Verified Customer | Posted 07/12/2018
  • Human IL-4 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: CHO Chinese hamster ovary cell line
    Verified Customer | Posted 04/24/2018

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FAQs

  • Why is plasma a validated sample type for the Human IL-4 Quantikine® ELISA (Catalog # D4050), but not in the high sensitivity version (Catalog # HS400)?

    The recovery and linearity obtained with plasma samples assayed with the high sensitivity kit HS400 show too much variability and did not meet our specifications, unlike the D4050 which shows reliable recovery and linearity data with plasma samples.

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.