Human IL-20 DuoSet ELISA

Name: Human IL-20 DuoSet ELISA
Brand: R&D Systems
SKU: DY1102

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY1102

Citations (2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human IL-20 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Human IL-20 DuoSet ELISA

Summary for Human IL-20 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-20. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human IL-20 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human IL-20 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

Scientific Data Examples for Human IL-20 DuoSet ELISA

	Human IL-20 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-20

Mature human and mouse IL-20 is a 152 amino acid protein that is not likely glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor-expressing cells.

Item	Value
Long Name	Interleukin 20
Entrez Gene IDs	50604 (Human); 58181 (Mouse)
Alternate Names	Cytokine Zcyto10, IL-20, IL-20ZCYTO10four alpha helix cytokine, IL10D, IL20, MGC96907, interleukin 20, interleukin-20

Citations for Human IL-20 DuoSet ELISA (2)

Showing 1 - 2 of 2 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human IL-20 DuoSet ELISA include:

Species: Human
Sample Types: Cell Lysates

HH Wang et al. (2022), Interleukin-20 is involved in dry eye disease and is a potential therapeutic target Journal of Biomedical Science, 29(1):36.
PMID: 35681232
Species: Human

GR Kathamuthu et al. (2018), Diminished circulating plasma and elevated lymph node culture supernatant levels of IL-10 family cytokines in tuberculous lymphadenitis Cytokine, 0(0).
PMID: 29871780

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Product Documents for Human IL-20 DuoSet ELISA

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS