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Human IL-2 DuoSet ELISA Best Seller

Catalog # DY202 | R&D Systems, Inc. a Bio-Techne Brand
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DY202
DY202-05

Ancillary Products Available

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

15.6-1000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human IL-2 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Product Summary for Human IL-2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Label

HRP

Scientific Data Images for Human IL-2 DuoSet ELISA

Human IL-2 ELISA Standard Curve

Human IL-2 ELISA Standard Curve

Kit Contents for Human IL-2 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Block Buffer: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Reagent Diluent: 0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha-helical polypeptide that is a member of the Common gamma Chain ( gammac) cytokine family (1-4). It exists as a monomer and has a notably short half-life (< 30 minutes) (1). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region (5, 6). The mature region is alpha-helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity (7). Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma delta T cells (8), activated conventional CD4+ and CD8+ T cells (1, 9), neurons (10, 11), microglia (12), and hematopoietic stem cells (13). 
The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gammac to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gammac also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3). 
In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).

Long Name

Interleukin 2

Alternate Names

Aldesleukin, IL2, Proleukin, TCGF

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Gene Symbol

IL2

Product Documents for Human IL-2 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-2 DuoSet ELISA

For research use only

Citations for Human IL-2 DuoSet ELISA (66)

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Customer Reviews for Human IL-2 DuoSet ELISA (12)

4.5 out of 5
12 customer ratings
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  • Human IL-2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 02/27/2023
  • Human IL-2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 01/27/2021
  • Human IL-2 DuoSet ELISA
    Name: Robert Berahovich
    Sample Tested: Tissue Culture Media
    Verified Customer | Posted 09/29/2020
    Detection of IL-2 produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.
    Human IL-2 DuoSet ELISA DY202
  • Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 09/26/2020
    Human IL-2 DuoSet ELISA DY202
  • Human IL-2 DuoSet ELISA
    Name: Liang Lin
    Sample Tested: Activated T cell
    Verified Customer | Posted 08/26/2020
    Human IL-2 DuoSet ELISA DY202
  • Human IL-2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 07/31/2020
    We used this kit for the quantification of IL-2 in the cell culture supernatant of human CAR T cells. The protocol is well described and the standard curve works well.
    Human IL-2 DuoSet ELISA DY202
  • Human IL-2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 04/02/2020
    We used this kit for the quantification of Il-2 in human serum and plasma. We could not detect Il-2 in our samples, but the standard curve works very well and well-described protocol.
    Human IL-2 DuoSet ELISA DY202
  • Human IL-2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: human primary T cells
    Verified Customer | Posted 12/11/2019
    Human IL-2 DuoSet ELISA DY202
  • Human IL-2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 05/06/2019
    Human IL-2 DuoSet ELISA DY202
  • Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 10/12/2018
    Human IL-2 DuoSet ELISA DY202
  • Human IL-2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 08/06/2018
  • Human IL-2 DuoSet ELISA
    Name: Shelley Anderson
    Sample Tested: Human cells
    Verified Customer | Posted 10/20/2017

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FAQs for Human IL-2 DuoSet ELISA

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  • Q: Is PBS a suitable alternative with which to formulate the Reagent Diluent?

    A: Our lab has determined that Tris-buffered saline generates optimal assay performance in comparison to PBS for this assay. This assay is validated with Reagent Diluent formulated using Tris-buffered saline.

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