Human IL-17 Quantikine QuicKit ELISANew

Name: Human IL-17 Quantikine QuicKit ELISA
Brand: rnd
SKU: QK317

Catalog # QK317

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	80 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	6 pg/mL
Assay Range:	31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human IL-17 Quantikine QuicKit ELISA Summary:	The Quantikine™ QuicKit Human IL-17 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IL-17 levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IL-17 and antibodies raised against the recombinant protein. Results obtained using natural human IL-17 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.

View Full Technical Details for Human IL-17 Quantikine QuicKit ELISA

Product Specifications

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IL-17.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	197	1025	201	1090
Standard Deviation	7.09	56.4	6.41	55.2
CV%	3.6	5.5	3.2	5.1

Recovery

The recovery of human IL-17 spiked to three levels in samples throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	112	106-118
EDTA Plasma (n=2)	115	107-122
Heparin Plasma (n=2)	110	106-116
Serum (n=2)	83	79-86

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-17 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human IL-17 Quantikine QuicKit ELISA Scientific Data Examples

	Human IL-17 Standard Curve QuicKit ELISA

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Item	Value
Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names	CTLA-8, CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17, IL17A, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A

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