Human IL-11 Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # D1100
Key Product Details
Assay Length
4.5 hours or 5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL)
Sensitivity
8 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates)
31.2-2000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
31.2-2000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human IL-11 Quantikine ELISA Kit
The Quantikine Human IL-11 Immunoassay is a 4.5-5.0 hour, solid-phase ELISA designed to measure IL-11 in cell culture supernates, serum, or plasma. It contains Sf 21-expressed recombinant human IL-11 and antibodies raised against recombinant human IL-11. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-11 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-11.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human IL-11
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 70.7 | 198 | 565 | 66.8 | 188 | 562 |
| Standard Deviation | 1.7 | 5.2 | 11.8 | 3.4 | 16.8 | 37.2 |
| CV% | 2.4 | 2.6 | 2.1 | 5.1 | 8.9 | 6.6 |
Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 50.0 | 320 | 1051 | 51.0 | 305 | 1018 |
| Standard Deviation | 2.2 | 15.3 | 50.9 | 4.1 | 11.4 | 73.8 |
| CV% | 4.4 | 4.8 | 4.8 | 8.0 | 3.7 | 7.2 |
Recovery for Human IL-11 Quantikine ELISA Kit
The recovery of human IL-11 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 98 | 90-108 |
| Citrate Plasma (n=5) | 105 | 96-118 |
| EDTA Plasma (n=5) | 103 | 97-110 |
| Heparin Plasma (n=5) | 103 | 93-114 |
| Serum (n=5) | 99 | 89-117 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-11 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human IL-11 Quantikine ELISA Kit
Human IL-11 ELISA Cell Culture Supernate Standard Curve
Human IL-11 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-11
Interleukin-11, also known as AGIF, is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27, and IL-31. It is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts. It is found in the plasma mainly during inflammation, and is considered to be primarily anti-inflammatory. It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine. It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis. IL-11 signals through a receptor complex that contains IL-11 R alpha and gp130. A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface or inhibit cell surface IL-11 R alpha/gp130 signaling.
Long Name
Interleukin 11
Alternate Names
AGIF, IL11, Oprelvekin
Gene Symbol
IL11
Additional IL-11 Products
Product Documents for Human IL-11 Quantikine ELISA Kit
Product Specific Notices for Human IL-11 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.