Human ICOS DuoSet ELISA

Name: Human ICOS DuoSet ELISA
Brand: R&D Systems
SKU: DY169

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY169

Citations (2)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	78.1 - 5,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human ICOS DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human ICOS DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ICOS. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human ICOS DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human ICOS DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human ICOS DuoSet ELISA

	Human ICOS ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ICOS

Inducible costimulator (ICOS), also called AILIM (activation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human and mouse ICOS share approximately 72% amino acid sequence identity. ICOS is expressed on most CD45RO+ cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand.

Item	Value
Long Name	Inducible Co-stimulator
Entrez Gene IDs	29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names	AILIM, AILIMCD278, Activation-inducible lymphocyte immunomediatory molecule, CD278, CD278 antigen, CRP-1, CVID1, ICOS, MGC39850, inducible T-cell co-stimulator, inducible T-cell costimulator, inducible costimulator

Citations for Human ICOS DuoSet ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human ICOS DuoSet ELISA include:

Species: Human
Sample Types: Serum

K McKinski et al. (2022), Orthogonal quantification of soluble inducible T-cell costimulator (ICOS) in healthy and diseased human serum Journal of pharmaceutical analysis, 12(2):317-323.
PMID: 35582393
Species: Human
Sample Types: Plasma

R Shivakoti et al. (2018), Inflammation and micronutrient biomarkers predict clinical HIV treatment failure and incident active TB in HIV-infected adults: a case-control study BMC Med, 16(1):161.
PMID: 30244671

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