Human Hyaluronidase 1/HYAL1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY7358
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
12.5-800 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Hyaluronidase 1/HYAL1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Hyaluronidase 1/HYAL1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Hyaluronidase 1 / HYAL1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Hyaluronidase 1/HYAL1 DuoSet ELISA
Human Hyaluronidase 1 / HYAL1 ELISA Standard Curve
Kit Contents for Human Hyaluronidase 1/HYAL1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Hyaluronidase 1/HYAL1
HYAL1 is one of five hyaluronidases with endo-beta-N-acetyl-hexosaminidase activity. It is a lysosomal enzyme with optimal activity around pH 4.0. It is highly expressed in the liver, kidney and heart, and it is the predominant hyaluronidase found in plasma. Defects in HYAL1 are associated with the hyaluronidase deficiency, mucopolysaccharidosis type IX. HYAL1 exhibits both tumor promoter and suppressor activity. While HYAL1, HYAL2, and SPAM1 are endo- beta-N-acetyl-glucosaminidases that primarily act on hyaluronan, HYAL4 is primarily active on chondroitin sulfate (types C and D in particular).
Long Name
Hyaluronoglucosaminidase-1
Alternate Names
FUS2, HYAL1, Hyaluronoglucosaminidase 1, LUCA1, NAT6
Gene Symbol
HYAL1
Additional Hyaluronidase 1/HYAL1 Products
Product Documents for Human Hyaluronidase 1/HYAL1 DuoSet ELISA
Product Specific Notices for Human Hyaluronidase 1/HYAL1 DuoSet ELISA
For research use only
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