Human Flt-3 Ligand/FLT3L DuoSet ELISA

Name: Human Flt-3 Ligand/FLT3L DuoSet ELISA
Brand: R&D Systems
SKU: DY308

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY308

Citations (5)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	15.6 - 1,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Flt-3 Ligand/FLT3L DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human Flt-3 Ligand/FLT3L DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Flt-3 Ligand. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human Flt-3 Ligand/FLT3L DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Flt-3 Ligand/FLT3L DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human Flt-3 Ligand/FLT3L DuoSet ELISA

	Human Flt-3 Ligand ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation.

Item	Value
Long Name	fms-like Tyrosine Kinase 3 Ligand
Entrez Gene IDs	2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)
Alternate Names	FL, FLG3L, FLT3LG, Flt-3 Ligand, Flt3 ligand, Flt3L, SL cytokine, fms-related tyrosine kinase 3 ligand

Citations for Human Flt-3 Ligand/FLT3L DuoSet ELISA (5)

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Citations are publications that use Bio-Techne products. Selected citations for Human Flt-3 Ligand/FLT3L DuoSet ELISA include:

Species: Human
Sample Types: Serum

P Peterlin et al. (2020), A new cytokine-based dynamic stratification during induction is highly predictive of survivals in acute myeloid leukemia Cancer Medicine, 0(0).
PMID: 33369136
Species: Human
Sample Types: Plasma

P Peterlin et al. (2019), FLT3 ligand plasma levels in acute myeloid leukemia Haematologica, 0(0).
PMID: 30630980
Species: Human
Sample Types: Plasma

P Peterlin et al. (2019), FLT3 ligand plasma levels have no impact on outcomes after allotransplant in acute leukemia Cytokine, 120(0):85-87.
PMID: 31035172
Species: Human
Sample Types: Plasma

Andersson S et al. (2012), Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis. PLoS ONE, 7(10):e47668.
PMID: 23082191
Species: Human
Sample Types: Recombinant Protein

Erlendsson LS et al. (2010), Barley as a green factory for the production of functional Flt3 ligand. Biotechnol J, 5(2):163-71.
PMID: 19844912

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Showing 1 - 5 of 5 citations Showing All

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