Human FGF-BP DuoSet ELISA

Name: Human FGF-BP DuoSet ELISA
Brand: R&D Systems
SKU: DY1593-05
Rating: 4 (2 reviews)

Catalog # DY1593-05 | R&D Systems, Inc. a Bio-Techne Brand

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Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	23.4 - 1,500 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human FGF-BP DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Technical Data
Citations (1) / Reviews (2)

Summary for Human FGF-BP DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human FGF-BP. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human FGF-BP DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human FGF-BP DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human FGF-BP DuoSet ELISA

	Human FGF-BP ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-BP

FGF binding protein (FGF-BP) was originally identified as a 17 kDa low-affinity heparin-binding protein in the conditioned medium of human A431 epidermoid carcinoma cells that bind FGF acidic and basic in a non-covalent, reversible manner. Rat FGF-BP cDNA encodes a 238 amino acid residue (aa) precursor protein with a 33 aa signal peptide. All FGF-BPs share ten conserved cysteine residues which form five intracellular disufide bridges.

Item	Value
Long Name	Fibroblast Growth Factor Binding Protein
Entrez Gene IDs	9982 (Human); 14181 (Mouse); 64535 (Rat)
Alternate Names	17 kDa heparin-binding growth factor-binding protein, FGF-BP, FGF-BP1, FGFBP, FGFBP-1, FGFBP1, FGFBPFGF-binding protein 1, HBP1717 kDa HBGF-binding protein, HBp17, fibroblast growth factor binding protein 1, fibroblast growth factor-binding protein 1, heparin-binding growth factor binding protein

Citations for Human FGF-BP DuoSet ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human FGF-BP DuoSet ELISA include:

Species: Human
Sample Types: Serum

X Chen et al. (2021-08-02), Poly-L-arginine promotes asthma angiogenesis through induction of FGFBP1 in airway epithelial cells via activation of the mTORC1-STAT3 pathway Cell Death Disease, 2021-08-02;12(8):761.
PMID: 34341336

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Customer Reviews for Human FGF-BP DuoSet ELISA (2)

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Human FGF-BP DuoSet ELISA

Name: Anonymous

Sample Tested: Serum and Plasma

Verified Customer | Posted 05/16/2022

We used this kit to quantify FGF-BP in human serum and plasma, need more serum/plasma sample.
Human FGF-BP DuoSet ELISA

Name: Jeff Pimentel

Sample Tested: Serum

Verified Customer | Posted 11/02/2018

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Product Documents for Human FGF-BP DuoSet ELISA

Product Datasheets

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Human FGF-BP DuoSet ELISA

Catalog # DY1593-05 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Human FGF-BP DuoSet ELISA

Product Specifications for Human FGF-BP DuoSet ELISA

Kit Contents for Human FGF-BP DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human FGF-BP DuoSet ELISA

Preparation and Storage

Background: FGF-BP

Citations for Human FGF-BP DuoSet ELISA (1)

Customer Reviews for Human FGF-BP DuoSet ELISA (2)

Customer Images

Human FGF-BP DuoSet ELISA

Human FGF-BP DuoSet ELISA

Product Documents for Human FGF-BP DuoSet ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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