Human FGF-23 DuoSet ELISA

Name: Human FGF-23 DuoSet ELISA
Brand: R&D Systems
SKU: DY2604-05

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY2604-05

Citations (4)

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	78.1 - 5,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human FGF-23 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human FGF-23 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human FGF-23. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human FGF-23 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human FGF-23 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human FGF-23 DuoSet ELISA

	Human FGF-23 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-23

FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.

Item	Value
Long Name	Fibroblast Growth Factor 23
Entrez Gene IDs	8074 (Human); 64654 (Mouse)
Alternate Names	ADHR, FGF-23, FGF23, HPDR2, HYPF, PHPTC, Tumor-derived hypophosphatemia-inducing factor, fibroblast growth factor 23, phosphatonin, tumor-derived hypophosphatemia inducing factor

Citations for Human FGF-23 DuoSet ELISA (4)

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Citations are publications that use Bio-Techne products. Selected citations for Human FGF-23 DuoSet ELISA include:

Species: Human
Sample Types: Serum

GT Chuang et al. (2020), Genome-wide association study for circulating fibroblast growth factor 21 and 23 Sci Rep, 10(1):14578.
PMID: 32884031
Species: Human
Sample Types: Serum

A Farías-Bas et al. (2019), Circulating Levels of Soluble Klotho and Fibroblast Growth Factor 23 in Mexican Diabetic Patients and Its Association With Early Nephropathy Arch. Med. Res., 0(0).
PMID: 30718148
Species: Human
Sample Types: Plasma

FM de Oliveir et al. (2019), Fibroblast growth factor 23, endothelium biomarkers and acute kidney injury in critically-ill patients J Transl Med, 17(1):121.
PMID: 30971270
Species: Human
Sample Types: Serum

G Deng et al. (2018), The Value of Older Donors' Klotho Level in Predicting Recipients' Short-Term Renal Function Med. Sci. Monit., 24(0):7936-7943.
PMID: 30396199

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