Human E-Cadherin Quantikine ELISA Kit

Name: Human E-Cadherin Quantikine ELISA Kit
Brand: rnd
SKU: DCADE0B

Catalog # DCADE0B

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
Sensitivity:	0.126 ng/mL
Assay Range:	0.625 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Human E-Cadherin Quantikine ELISA Kit Summary:	The Quantikine™ Human E-Cadherin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human E-Cadherin in cell culture supernates, serum, plasma, saliva, and urine. It contains HEK293-expressed recombinant human E-Cadherin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human E-Cadherin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human E-Cadherin.

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Product Specifications

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human E-Cadherin.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.42	7.06	14.6	2.19	6.55	14.2
Standard Deviation	0.047	0.145	0.266	0.136	0.368	0.845
CV%	1.9	2.1	1.8	6.2	5.6	6

Recovery

The recovery of human E-Cadherin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=30)	242	131-479
Heparin Plasma (n=30)	236	116-479
Saliva (n=10)	145	21.4-278
Serum (n=30)	250	127-492
Urine (n=10)	60	3.03-121

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human E-Cadherin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human E-Cadherin Quantikine ELISA Kit Scientific Data Examples

	Human E-Cadherin ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Cadherin

The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2⁺-dependent cell-cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin.

Item	Value
Entrez Gene IDs	999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names	Arc-1, CAD1, CAM 120/80, CD324, CD324 antigen, CDH1, CDHE, Cadherin-1, Cell-CAM120/80, E-Cadherin, ECAD, ECadherin, Epithelial cadherin, L-CAM, LCAM, UVOE-Cadherin, Uvomorulin, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), calcium-dependent adhesion protein, epithelial, cell-CAM 120/80

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