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Human Cyr61/CCN1 Quantikine ELISA Kit

Bio-Techne includes R&D Systems | Catalog # DCYR10

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DCYR10

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Saliva (50 µL), Human Milk (10 µL)

Sensitivity

3.8 pg/mL

Assay Range

39.1-2500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Saliva, Human Milk)

Product Summary for Human Cyr61/CCN1 Quantikine ELISA Kit

The Quantikine Human Cyr61/CCN1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Cyr61 in cell culture supernates, serum, plasma, saliva, and human milk. It contains CHO cell-expressed recombinant human Cyr61 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Cyr61 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Cyr61.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human Cyr61

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Human Milk, Saliva, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 256 721 1442 249 712 1445
Standard Deviation 5.94 14.2 33.5 15.9 35.0 73.3
CV% 2.3 2.0 2.3 6.4 4.9 5.1

Recovery for Human Cyr61/CCN1 Quantikine ELISA Kit

The recovery of Cyr61 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 98-117
EDTA Plasma (n=4) 98 91-104
Serum (n=4) 101 95-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cyr61 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Cyr61/CCN1 ELISA Linearity

Scientific Data Images for Human Cyr61/CCN1 Quantikine ELISA Kit

Human Cyr61/CCN1 ELISA Standard Curve

Human Cyr61/CCN1 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cyr61/CCN1

Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa matricellular glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis through interactions with integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Entrez Gene IDs

3491 (Human); 16007 (Mouse); 83476 (Rat)

Gene Symbol

CCN1

Additional Cyr61/CCN1 Products

Product Documents for Human Cyr61/CCN1 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Cyr61/CCN1 Quantikine ELISA Kit

For research use only

Citations for Human Cyr61/CCN1 Quantikine ELISA Kit (5)

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  • Human Cyr61/CCN1 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 02/17/2020
    Precise and Accurate results. Easy to use kit.
    Human Cyr61/CCN1 Quantikine ELISA Kit DCYR10

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.