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Human CXCL4/PF4 Quantikine ELISA Kit

Catalog # DPF40 | R&D Systems, Inc. a Bio-Techne Brand
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DPF40

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL)

Sensitivity

0.1 ng/mL

Assay Range

0.781-50 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Product Summary for Human CXCL4/PF4 Quantikine ELISA Kit

The Quantikine Human CXCL4/PF4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PF4 in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PF4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PF4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PF4.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human PF4.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 5.80 14.1 26.8 5.61 14.3 27.2
Standard Deviation 0.472 0.840 1.85 0.558 1.70 3.20
CV% 8.1 6.0 6.9 9.9 11.9 11.8

Recovery for Human CXCL4/PF4 Quantikine ELISA Kit

The recovery of human PF4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 110 99-117
Platelet-poor EDTA Plasma (n=4) 100 89-117

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human PF4 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CXCL4/PF4 ELISA Linearity

Scientific Data Images for Human CXCL4/PF4 Quantikine ELISA Kit

Human CXCL4/PF4 ELISA Standard Curve

Human CXCL4/PF4 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL4/PF4

CXCL4/PF4 is a secreted chemokine that is expressed by megakaryocytes and restrains the coagulation cascade. It is released by activated platelets and regulates Thrombin/Thrombomodulin complexes, activated Protein C (APC), Coagulation Factor Xa, and Fibrin. The anticoagulant heparin neutralizes CXCL4 procoagulant effects. Complexes of heparin and CXCL4 trigger antibody formation that causes the pathological syndrome HITT. Immunogenic complexes of CXCL4 with Apolipoprotein H contribute to antiphospholipid syndrome (APS). CXCL4 interferes with the proliferative and angiogenic functions of FGF-2 and VEGF. However, it can also exert proinflammatory and pro-atherogenic effects on monocytes, macrophages, and endothelial cells. CXCL4 may signal through CXCR3B in humans.

Alternate Names

PF4

Entrez Gene IDs

5196 (Human); 56744 (Mouse); 360918 (Rat)

Gene Symbol

PF4

Product Documents for Human CXCL4/PF4 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCL4/PF4 Quantikine ELISA Kit

For research use only

Citations for Human CXCL4/PF4 Quantikine ELISA Kit (15)

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Customer Reviews for Human CXCL4/PF4 Quantikine ELISA Kit (2)

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  • Human CXCL4/PF4 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Recombinant protein
    Verified Customer | Posted 09/14/2019
  • Human CXCL4/PF4 Quantikine ELISA Kit
    Name: Balaji Mahender
    Sample Tested: EDTA Plasma and Citrate Plasma
    Verified Customer | Posted 01/09/2018

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Setting the standard in quality research reagents

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.