Human Chymase/CMA1 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY4099-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
93.8-6000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Chymase/CMA1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Chymase/CMA1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Chymase. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as tissue lysate, serum, and plasma should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Chymase/CMA1 DuoSet ELISA
Human Chymase ELISA Standard Curve
Kit Contents for Human Chymase/CMA1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Chymase/CMA1
Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells. They are synthesized as inactive precursors containing a 2-residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for enzymatic activity. Human chymase encoded by the CMA1 gene is known to be involved in hypertension and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II). In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines.
Alternate Names
CMA1, CYH, MCP3P, Mcpt5, MCT1
Gene Symbol
CMA1
Additional Chymase/CMA1 Products
Product Documents for Human Chymase/CMA1 DuoSet ELISA
Product Specific Notices for Human Chymase/CMA1 DuoSet ELISA
For research use only
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