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Human CCL28 DuoSet ELISA

Bio-Techne includes R&D Systems | Catalog # DY717

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DY717

Ancillary Products Available

Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

62.5-4000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human CCL28 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Product Summary for Human CCL28 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL28. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Label

HRP

Scientific Data Images for Human CCL28 DuoSet ELISA

Human CCL28 ELISA Standard Curve

Human CCL28 ELISA Standard Curve

Kit Contents for Human CCL28 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Block Buffer: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Reagent Diluent: 0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL28

Human CCL28 (CC chemokine ligand 28) is a member of the CC chemokine family. Human and mouse CCL28 are highly conserved, sharing 83% amino acid sequence identity in their mature regions. CCL28 RNA expression is highest in normal and pathologic colon epithelial cells. Human CCL28 RNA is also present in normal and asthmatic lung tissues. The receptor for CCL28 is CCR10 (GPR2 orphan receptor), which is also the receptor for CCL27/CTACK .

Alternate Names

CCL28, MEC, VIC

Entrez Gene IDs

56477 (Human); 56838 (Mouse)

Gene Symbol

CCL28

Additional CCL28 Products

Product Documents for Human CCL28 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CCL28 DuoSet ELISA

For research use only

Citations for Human CCL28 DuoSet ELISA (5)

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Customer Reviews for Human CCL28 DuoSet ELISA (1)

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  • Human CCL28 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 04/02/2020
    We used this kit for the quantification of CCL28 in human serum and plasma. Works very well and well-described protocol.
    Human CCL28 DuoSet ELISA DY717

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human CCL28 DuoSet ELISA

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  • Q: Is PBS a suitable alternative with which to formulate the Reagent Diluent?

    A: Our lab has determined that Tris-buffered saline generates optimal assay performance in comparison to PBS for this assay. This assay is validated with Reagent Diluent formulated using Tris-buffered saline.

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