Human B7-H3 Quantikine ELISA Kit

Name: Human B7-H3 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DB7H30

Catalog # DB7H30 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)
Sensitivity:	0.274 ng/mL
Assay Range:	0.781 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Human B7-H3 Quantikine ELISA Kit Summary:	The Quantikine Human B7-H3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human B7-H3 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human B7-H3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human B7-H3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human B7-H3.

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Technical Data
Citations (2) / Reviews

Product Specifications for Human B7-H3 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human B7-H3
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	5.89	19	37.5	5.88	17.6	35.1
Standard Deviation	0.2	0.6	0.9	0.3	0.6	1.7
CV%	3.4	3.3	2.5	5.1	3.5	5

Recovery

The recovery of B7-H3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	105	99-112
EDTA Plasma (n=4)	104	91-115
Heparin Plasma (n=4)	102	93-114
Serum (n=4)	103	94-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of B7-H3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human B7-H3 Quantikine ELISA Kit

	Human B7-H3 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: B7-H3

B7-H3 is an immunoglobulin superfamily protein that is expressed by osteoblasts, fibroblasts, ciliated respiratory epithelial cells, anterior pituitary progenitor cells, first trimester extravillous trophoblasts, and multiple tumor cells. It is induced on activated dendritic cells (DC), skeletal muscle myocytes, and monocytes. B7-H3 can both stimulate and inhibit immune cell activation. As a co-stimulator, B7-H3 augments macrophage activation and promotes proliferation and cytokine secretion by CD4+ and CD8+ T cells. As a co-inhibitor, tumor cell B7-H3 blocks the cytolytic activity of NK cells, limits cytokine production by activated T cells, and imparts an immunosuppressive phenotype to dendritic cells. B7-H3 can interact with TREML2/TLT-2 to enhance T cell activation including the CD8+ T cell-mediated lysis of tumor cells.

Item	Value
Long Name	B7 Homolog 3
Entrez Gene IDs	80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names	B7-H3, B7-H3B7 homolog 3, B7H3, B7H34Ig-B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule

Citations for Human B7-H3 Quantikine ELISA Kit (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human B7-H3 Quantikine ELISA Kit include:

Species: Human
Sample Types: Bile

Y Liu et al. (2019), Value of bile soluble B7H3 for the diagnosis of malignant biliary strictures: Results of a retrospective study Surg Oncol, 28(0):195-200.
PMID: 30851900
Species: Human
Sample Types: Cell Culture Supernates

Chao Xie (2016), Soluble B7-H3 promotes the invasion and metastasis of pancreatic carcinoma cells through the TLR4/NF-?B pathway Sci Rep, 6(0):27528.
PMID: 27273624

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Product Documents for Human B7-H3 Quantikine ELISA Kit

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SDS

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.