Human Axl Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DAXL00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (50 µL), Urine (10 µL), Cerebrospinal Fluid (10 µL)
Sensitivity
4.6 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)
Product Summary for Human Axl Quantikine ELISA Kit
The Quantikine® Human Axl Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Axl in cell culture supernates, cell lysates, cerebrospinal fluid, serum, plasma, saliva, and urine. It contains
NS0-expressed recombinant human Axl and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Axl showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Axl.
NS0-expressed recombinant human Axl and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Axl showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Axl.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Axl
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates, Cell Lysates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 249 | 565 | 1115 | 258 | 582 | 1131 |
| Standard Deviation | 4.21 | 10.0 | 18.6 | 11.3 | 19.4 | 42.4 |
| CV% | 1.7 | 1.8 | 1.7 | 4.4 | 3.3 | 3.7 |
Recovery for Human Axl Quantikine ELISA Kit
The recovery of human Axl spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 89 | 81-98 |
| Cell Lysis Buffer (n=1) | 90 | 88-93 |
| Saliva (n=4) | 94 | 82-101 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Axl were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Axl Quantikine ELISA Kit
Human Axl Quantikine ELISA Kit Linearity Competitor Comparison
Human Axl ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Axl
Axl, also known as Ufo and Ark, is a widely expressed glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis. Axl binds the vitamin K-dependent protein Gas6, triggering platelet aggregation and thrombus formation, macrophage and dendritic cell phagocytosis of apoptotic cells, NK cell development from hematopoietic progenitor cells, and in vivo angiogenesis. Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness. It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow. It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses.
Long Name
Axl Receptor Tyrosine Kinase
Alternate Names
AI323647, ARK, JTK11, Tyro7, UFO
Gene Symbol
AXL
Additional Axl Products
Product Documents for Human Axl Quantikine ELISA Kit
Product Specific Notices for Human Axl Quantikine ELISA Kit
For research use only
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