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Human Angiopoietin-like 3 Quantikine ELISA Kit Best Seller

Catalog # DANL30 | R&D Systems, Inc. a Bio-Techne Brand
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DANL30

Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.035 ng/mL

Assay Range

0.156-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Product Summary for Human Angiopoietin-like 3 Quantikine ELISA Kit

The Quantikine Human ANGPTL3 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human ANGPTL3 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human ANGPTL3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ANGPTL3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ANGPTL3.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural and recombinant human ANGPTL3

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.05 3.36 6.80 1.02 3.18 6.74
Standard Deviation 0.04 0.09 0.18 0.07 0.20 0.60
CV% 4.1 2.7 2.7 6.7 7.2 8.5

Recovery for Human Angiopoietin-like 3 Quantikine ELISA Kit

The recovery of ANGPTL3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 99-107
EDTA Plasma (n=4) 102 85-120
Heparin Plasma (n=4) 96 85-109
Serum (n=4) 96 81-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of ANGPTL3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Angiopoietin-like Protein 3/ANGPTL3 ELISA Linearity

Scientific Data Images for Human Angiopoietin-like 3 Quantikine ELISA Kit

Human Angiopoietin-like Protein 3/ANGPTL3 ELISA Standard Curve

Human Angiopoietin-like Protein 3/ANGPTL3 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

Product Documents for Human Angiopoietin-like 3 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Angiopoietin-like 3 Quantikine ELISA Kit

For research use only

Citations for Human Angiopoietin-like 3 Quantikine ELISA Kit (8)

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Customer Reviews for Human Angiopoietin-like 3 Quantikine ELISA Kit (2)

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  • Human Angiopoietin-like 3 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 08/09/2023
    Human Angiopoietin-like 3 Quantikine ELISA Kit DANL30
  • Human Angiopoietin-like 3 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 05/26/2022
    Human Angiopoietin-like 3 Quantikine ELISA Kit DANL30

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Setting the standard in quality research reagents

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.