Human Adiponectin/Acrp30 DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY1065
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
62.5-4000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Adiponectin/Acrp30 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Adiponectin/Acrp30 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Adiponectin / Acrp30. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Adiponectin/Acrp30 DuoSet ELISA
Human Adiponectin / Acrp30 ELISA Standard Curve
Kit Contents for Human Adiponectin/Acrp30 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent:1% BSA in PBS, pH 7.2 - 7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).
Blocking Buffer:1% BSA in PBS, pH 7.2 - 7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Adiponectin/Acrp30
Adiponectin (Acrp30) is secreted primarily by adipocytes and induces multiple paracrine and endocrine effects on metabolism and inflammation. Adiponectin promotes insulin sensitivity, decreased serum glucose and triglyceride levels, and increased serum glucagon levels. It promotes fatty acid uptake and oxidation, glucose uptake, and lactate production in skeletal muscle. Adiponectin promotes the development of M2 macrophages and promotes the C1qRp mediated clearance of apoptotic cell debris. Decreased plasma HMW Adiponectin levels are associated with upper body obesity, insulin resistance, reduced fatty acid oxidation, dyslipidemia, coronary artery disease, and atherogenesis. Adiponectin forms homotrimers, hexamers, and high molecular weight (HMW) complexes and signals through the receptors AdipoR1 and AdipoR2.
Alternate Names
Acrp30, AdipoQ, ApM1, GBP28
Gene Symbol
ADIPOQ
Additional Adiponectin/Acrp30 Products
Product Documents for Human Adiponectin/Acrp30 DuoSet ELISA
Product Specific Notices for Human Adiponectin/Acrp30 DuoSet ELISA
For research use only
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