One of our customer would be interested in your kits: Dopamine ELISA Kit (KA1887) and Epinephrine ELISA Kit (KA1882). The customer is going to test mouse serum and plasma samples. Please, let me know if these kits could be use with mouse samples.

Both of these kits can be used with mouse urine and plasma samples very successfully.

I wanted some information regarding the ELISA kit form Novus biologicals cat no. KA1887 and NB100-64534. Its sensitivity and detection towards lowest levels of dopamine and cross reactivity. Can this product be used for quantifying DOPAMINE from culture supernatant?

KA1887 has been tested on urine and plasma and the limit of detection is 4.5 ng/mL urine and 20 pg/mL plasma. It will most likely work on cell culture supernatant as well. NB100-64534 is just an antibody that you would need to use to create your own ELISA, so the limit of detection has not been determined.

Will this ELISA work for tissue homogenates?

Thank you for contacting Novus Biologicals. KA1887 is made by Abnova, for whom we distribute in the US. This kit has been QC'd using plasma and urine. Abnova has not tested this for other samples and does not guarantee it for use on those samples. I have included a link to the protocol for your perusal. KA1887 Protocol.

Dopamine ELISA Kit (Colorimetric)

Name: Dopamine ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA1887

Novus Biologicals, part of Bio-Techne | Catalog # KA1887

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Plasma, Urine (Extracted urine samples: 25 uL, Extracted plasma samples: 50 uL)

Sensitivity

Urine: 2.5 ng/mL, Plasma: 49 pg/mL

Assay Range

Urine: 4.8 to 2000 ng/mL, Plasma: 75 to 33,333 pg/mL

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Product Specifications

Assay Type

Quantification

Kit Type

ELISA Kit (Colorimetric)

Description

Quality control test: Standard curve. Dopamine ELISA Kit is an enzyme immunoassay for the quantitative determination of Dopamine in plasma and urine.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 12.65%

Inter-Assay Precision (Precision between assays) CV% < 17.05%

Recovery for Dopamine ELISA Kit (Colorimetric)

Recovery

Urine: 95%, Plasma: 90%

Scientific Data Images for Dopamine ELISA Kit (Colorimetric)

ELISA: Dopamine ELISA Kit (Colorimetric) [KA1887]

ELISA: Dopamine ELISA Kit [KA1887] - Standard Curve Note: The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for Dopamine ELISA Kit (Colorimetric)

Acylation Buffer: Ready to use, buffer with light alkaline pH for the acylation.
Acylation Reagent: Ready to use. Acylation reagent in DMF and DMSO. H225 Highly flammable liquid and vapour. H360 May damage fertility or the unborn child. H319 Causes serious eye irritation.
Adhesive Foil: Ready to use, in a resealable pouch.
Adjustment Buffer: Ready to use, TRIS buffer.
Assay Buffer: Ready to use, 1 M hydrochloric acid and a non-mercury preservative.
Coenzyme: Ready to use, S-adenosyl-L-methionine
Dopamine Antiserum: Ready to use, rabbit anti-dopamine antibody, green coloured.
Dopamine Microtiter Strips: Ready to use, antigen precoated microwell plate in a resealable green pouch with desiccant.
Enzyme Conjugate: Ready to use, goat anti-rabbit immunoglobulins, conjugated with peroxidase.
Enzyme: Lyophilized Catechol-O-methyltransferase.
Extraction Buffer: Ready to use, buffer containing carbonate.
Extraction Plate: Ready to use, plates coated with boronate affinity gel in a resealable pouch.
Hydrochloric Acid: Ready to use, 0.025 M hydrochloric acid, yellow coloured.
Stop Solution: Ready to use, containing 0.25 M sulfuric acid.
Substrate: Ready to use, chromogenic substrate containing tetramethylbenzidine, substrate buffer and hydrogen peroxide.
Wash Buffer Concentrate (50x): Concentrated buffer with a non-ionic detergent and physiological pH.

Preparation and Storage

Formulation

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Dopamine

Dopamine is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.

Alternate Names

3 4 dihydroxyphenethylamine, 3 hydroxytyramine, DA, Dopamine, Oxytyramine

Additional Dopamine Products

Product Documents for Dopamine ELISA Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download Manual - Dopamine ELISA Kit (Colorimetric)

COA

Product Specific Notices for Dopamine ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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