CXCL5/ENA-78 Antibody Pair [Biotin] New
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57724
Key Product Details
Assay Type
Sandwich ELISA
Sample Type
Cell culture supernatants from mice (Mus musculus). Can also be used to measure CXCL5 in a variety of biological sample types reported in the literature, including serum, plasma, whole blood, and tissue lysates from organs such as lung, spleen, liver, and bone marrow. While the kit is optimized for cell culture supernatants, minor modifications to the protocol may be required when in other sample types to ensure optimal sensitivity and accuracy, allowing researchers to adjust buffers, antibody concentrations, incubation times, temperatures, and other assay conditions as needed. (100 ul)
Reactivity
Mouse
Product Specifications
Description
The kit contains a capture antibody, detection antibody, and recombinant protein standard for assay development, and these antibodies have been validated to function together in an ELISA format using the provided standard. The quantities of kit components are not matched, and individual components may be purchased separately. This product is for research use only.
Sample Volume Required
100 ul
Conjugate
Biotin
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: CXCL5/ENA-78
CXCL5/Epithelial Cell-derived Neutrophil-activating Peptide (ENA-78), is a member of the CXC subfamily of chemokines. Full-length CXCL5/ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. Following the removal of the signal peptide, bioactive CXCL5/ENA-78 is 78 aa in length. CXCL5/ENA-78 can be N-terminally cleaved by Cathepsin G and Chymotrypsin to CXCL5/ENA-74 (74 aa) and CXCL5/ENA-70 (70 aa), which show increased potency relative to CXCL5/ENA-78. While murine LIX was thought to be an ortholog to human CXCL5/ENA-78, genome-wide analysis and a consensus in the field suggests that human CXCL5/ENA-78 does not have a true murine ortholog. CXCL5/ENA-78 is upregulated at sites of inflammation and is expressed by multiple hematopoietic cell types, fibroblasts, endothelial cells, vascular smooth muscle cells, and adipocytes. It acts as a chemoattractant for neutrophils, has angiogenic properties, and contributes to cancer progression.
Alternate Names
CXCL5, ENA-78, ENA78
Gene Symbol
CXCL5
Additional CXCL5/ENA-78 Products
Product Documents for CXCL5/ENA-78 Antibody Pair [Biotin]
Product Specific Notices for CXCL5/ENA-78 Antibody Pair [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.
Loading...
Loading...
Loading...