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25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

Catalog # NBP2-66361 | Novus Biologicals a Bio-Techne Brand
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NBP2-66361

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (50 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

Product Specifications

Assay Type

Competitive-ELISA

Kit Type

ELISA Kit (Colorimetric)

Specificity

This kit recognizes 25-hydroxy Vitamin D3 in samples. No significant cross-reactivity or interference between 25-hydroxy Vitamin D3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.02%

Inter-Assay Precision (Precision between assays) CV% < 4.96%

Recovery for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

Recovery

87-112%

Linearity

25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

Scientific Data Images for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

Universal 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric) [NBP2-66361] - Standard Curve Reference

Kit Contents for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100×)
  • Concentrated HRP Conjugate (100×)
  • Concentrated Wash Buffer (25×)
  • HRP Conjugate Diluent
  • Micro ELISA Plate(Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 25-hydroxy Vitamin D3

Alternate Names

25-Hydroxycholecalciferol, 25-Hydroxyvitamin D, 25-hydroxyvitamin D3, 25-OH D3, 25-OH Vitamin D3

Product Documents for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 25-hydroxy Vitamin D3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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