Clozapine N-oxide (CNO)
Tocris Bioscience, part of Bio-Techne | Catalog # 4936
Activator of hM3Dq and hM4Di DREADDs
Key Product Details
Description
Activator of hM3Dq and hM4Di DREADDs
Alternative Names
CNO
Product Description
Clozapine N-oxide is a synthetic ligand for human muscarinic engineered receptors, designer receptors activated by designer drugs (DREADD). Binds and activates hM3Dq and hM4Di DREADDs in vitro and in vivo. Metabolite of Clozapine (Cat. No. 0444). Shown to be a P-glycoprotein (P-gp) efflux pump substrate.Example Applications of Clozapine N-oxide (CNO):
- Silences hippocampal neurons expressing hM4Di DREADDs in vitro
- Inhibits locomotor activity of mice expressing hM3Dq in GABAergic VTA neurons
- Inhibits short term memory retrieval in mice expressing hM4Di in hippocampal neurons
- Induces heat and mechanical hypersensitivity in mice expressing hM4Di in enkephalinergic neurons in the right lumbar dorsal horn
- Inhibits markers of social aggression in mice expressing hM4Di in hippocampal CA2 neurons
Alternative potent and selective hM3Dq and hM4Di DREADD agonists available: Deschloroclozapine and DREADD agonist 21
Water soluble salt also available.
This product contains 1 molar equivalent of ethanol.
Product Specifications
Molecular Weight
342.82
Formula
C18H19ClN4O
Storage
Store at RT
Purity
≥98% (HPLC)
Chemical Name
8-Chloro-11-(4-methyl-4-oxido-1-piperazinyl)-5H-dibenzo[b,e][1,4]diazepine
CAS Number
34233-69-7
PubChem ID
2819
InChI Key
WYRDWWAASBTJLM-UHFFFAOYSA-N
SMILES
ClC(C=C3)=CC1=C3NC(C=CC=C2)=C2C(N4CC[N+](C)([O-])CC4)=N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.86 | 20 |
Preparing Stock Solutions
for Clozapine N-oxide (CNO)
The following data is based on the product molecular weight 342.82
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 14.58 mL | 72.92 mL | 145.85 mL |
| 1 mM | 2.92 mL | 14.58 mL | 29.17 mL |
| 2 mM | 1.46 mL | 7.29 mL | 14.58 mL |
| 10 mM | 0.29 mL | 1.46 mL | 2.92 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Roth DREADDs for Neuroscientists. Neuron 2016 PMID: 26889809
- Nakajima Gs-coupled GPCR signalling in AgRP neurons triggers sustained increase in food intake. Nat.Commun. 2016 PMID: 26743492
- Gomez Chemogenetics revealed: DREADD occupancy and activation via converted cloz. Science 2017 PMID: 28774929
- Zaman LMO4 is essential for paraventricular hypothalamic neuronal activity and calcium channel expression to prevent hyperphagia. J.Neurosci. 2014 PMID: 24381275
- Armbruster Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand. Proc.Natl.Acad.Sci.U.S.A. 2007 PMID: 17360345
- Eiermann The involvement of CYP1A2 and CYP3A4 in the metabolism of cloz. Br.J.Clin.Pharmacol. 1997 PMID: 9384460
Product Specific Notices for Clozapine N-oxide (CNO)
For research use only
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