Human Erythrocyte Lysing Kit

For the removal of erythrocytes (red blood cells) from whole blood.

Key Benefits

• Gently lyses erythrocytes
• Stabilizes leukocytes for analysis by flow cytometry
• Maintains natural light scattering and fluorescent staining properties of leukocytes
• Maintains leukocyte viability for subsequent cell culture manipulation

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Why Remove Erythrocytes?

The lysis of erythrocytes from whole blood is an important initial step in the isolation and analysis of enriched leukocyte preparations. Recovered immune cells can be accurately characterized following red blood cell removal. Lysis of erythrocytes under conditions that do not disrupt lymphocytes or myeloid cells is critical for downstream applications utilizing leukocytes harvested from whole blood.

The Human Erythrocyte Lysing Kit contains the following reagents for the controlled lysis of erythrocytes in whole blood.

• H-Lyse Buffer Concentrate (10X)
• Wash Buffer Concentrate (10X)
• Fixative Concentrate (10X)

*This kit contains sufficient reagents to process 250 mL of whole blood.

Stability and Storage

Store all reagents at 20 °C to 25 °C. 

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Lysis of Erythrocytes in Whole Blood. Forward light scatter (FSC-H) vs. side light scatter (SSC-H) dot histograms of human blood before (A) and after (B) treatment with the Human Erythrocyte Lysing Kit. (C) Dot histogram of whole blood stained with PE-conjugated Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # FAB3832P) and APC-conjugated Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # FAB100A) followed by erythrocyte lysis using the Human Erythrocyte Lysing Kit. Stained cells were stored for 24 hours at 2 °C to 8 °C prior to flow cytometric analysis.