Specially formulated and optimized for tumorsphere formation
Why Perform the Tumorsphere Assay Using Specially Formulated Media?
Tumorsphere assays, also called sphere-formation assays, are in vitro assays that assess stem cell characteristics of tumor cells. There are a number of variables that contribute to variation in data obtained from tumorsphere assays including the purity of the starting cell population, the type and quantity of media and supplements, and the potential for tumorspheres to aggregate.
To minimize experimental variability due to the media and supplements, R&D Systems offers StemXVivo® Serum-Free Tumorsphere Media, which is formulated and optimized for tumorsphere formation.
StemXVivo® Serum-Free Tumorsphere Media:
Shown to support sphere formation of seven tumor cell lines.
Formulated and optimized for efficient tumorsphere formation.
100 mL of StemXVivo® Serum-Free Tumorsphere Media.
Stability and Storage
Upon receipt, StemXVivo® Serum-Free Tumorsphere Media should be stored at =-20 °C in a manual defrost freezer. The media can be thawed at 2 °C to 8 °C or at room temperature. Thawed media can be aliquoted and stored at =-20°C in a manual defrost freezer for up to 3 months or used within two weeks when stored in the dark at 2 °C to 8 °C. Avoid repeated freeze-thaw cycles. Do not use this product beyond the expiration date.
This product contains human transferrin. The transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, this product should be handled as if capable of transmitting infection.
FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
This reagent should not be used beyond the expiration date indicated on the label.
Results may vary with cells cultured by different methods.
As for all stem cells, oncogenically transformed cancer stem cells (CSCs) are defined by their ability for self-renewal and multipotency. The CSC hypothesis states that, although CSCs represent a rare population of cells within a tumor, their high tumorigenic capacity drives tumorigenesis. Due to their intrinsic stem cell-like properties, CSC proliferation generates more CSCs, and all the differentiated cell types that compose the bulk of the tumor. Non-CSCs in the tumor have been shown to proliferate at a faster rate than CSCs, but have little tumor-initiating potential. Because CSCs exhibit increased resistance to toxic and chemical insults, this specific subpopulation of cells is believed to underlie resistance to chemotherapy and disease relapse. In fact, the CSC model posits that all CSCs must be eradicated to eliminate a tumor and prevent its recurrence.
Product Specifications for StemXVivo Serum-Free Tumorsphere Media
Scientific Data Examples for StemXVivo Serum-Free Tumorsphere Media
Tumorspheres Formed from the MCF-7 Human Breast Cancer Cell Line.
MCF-7 human breast cancer cells were suspended in StemXVivo®Serum-Free Tumorsphere Media (Catalog # CCM012) containing 2 U/mL Heparin (Tocris Catalog # 2812) and 0.8 µg/mL Hydrocortisone (Tocris, Catalog # 4093). The cells were plated at 3 x 104cells per well in a 6-well ultralow adhesion culture plate and cultured at 37°C and 5% CO2for 3-10 days to induce tumorsphere formation. Tumorspheres were imaged using a CloneSelect™Imager from Molecular Devices (New Milton) Limited.
Preparation & Storage
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Refer to the product datasheet for complete product details.
Briefly, tumorsphere formation can be induced using the following procedure
Cells are suspended in StemXVivo Serum-Free Tumorsphere Media containing Heparin and Hydrocortisone
Cells are cultured on low adhesion plates at 37 °C and 5% CO2
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics