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StemXVivo Serum-Free Tumorsphere Media

Catalog # CCM012 | R&D Systems, Inc. a Bio-Techne Brand
For Tumorsphere Formation of Human/Mouse/Rat Cancer Cells

Key Product Details

Tumorspheres Formed from the MCF-7 Human Breast Cancer Cell Line.
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, tumorsphere formation can be induced using the following procedure

  • Cells are suspended in StemXVivo Serum-Free Tumorsphere Media containing Heparin and Hydrocortisone
  • Cells are cultured on low adhesion plates at 37 °C and 5% CO2
  • Tumorspheres should form in 3-10 days
 

 

Reagents Provided

Reagents supplied in the StemXVivo® Serum-Free Tumorsphere Media (Catalog # CCM012):

  • 100 mL of StemXVivo® Serum-Free Tumorsphere Media

 

Other Supplies Required

Reagents

  • Heparin (Tocris Catalog # 2812)
  • Hydrocortisone (Tocris Catalog # 4093)
  • Cell dissociation solution

Materials

  • 15 mL conical tube
  • Ultralow adhesion cell culture plates

Equipment

  • 37 °C and 5% CO2 incubator
  • Benchtop Centrifuge

 

Procedure Overview

Cryopreservation of Stem Cells

Add 2 U/mL Heparin and 0.5 µ;g/mL Hydrocortisone to pre-warmed StemXVivo® Serum-Free Tumorsphere Media.

Add 2 U/mL Heparin and 0.5 ug/mL Hydrocortisone to pre-warmed StemXVivo Serum-Free Tumorsphere Media.

Resuspend cells in the supplemented media.

Resuspend cells in the supplemented media.

Transfer the cell suspension to ultralow adhesion plates.

Transfer the cell suspension to ultralow adhesion plates.

Incubate cultures in a 5% CO2 and 37 °C incubator.

Tumorspheres should form in 3-10 days.

Tumorspheres should form in 3-10 days.

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FAQs for StemXVivo Serum-Free Tumorsphere Media

  • Can a 96-well ultra-low adhesion plate be used for Tumorsphere formation using Catalog # CCM012 (StemXVivo Serum-free Tumorsphere Media)?  How many cells should be plated /well?

    Yes. A 96-well ultra-low adhesion plate can be used for Tumorsphere formation.  A recommended cell number is approximately 100 cells/well, but it is advisable to titrate and evaluate the optimal cell number.

Product Documents for StemXVivo Serum-Free Tumorsphere Media

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.