Hydrocortisone

Name: Hydrocortisone
Brand: Tocris Bioscience
SKU: 4093
Rating: 4 (1 reviews)

Catalog # 4093 | Tocris Bioscience a Bio-Techne Brand

(1)

Citations (4)

COA / SDS

Adrenal glucocorticoid; immunosuppressant

Key Product Details

Description:	Adrenal glucocorticoid; immunosuppressant
Chemical Name:	11β,17α,21-Trihydroxypregn-4-ene-3,20-dione
Purity:	≥98% (HPLC)
Molecular Weight:	362.46

View Full Technical Details for Hydrocortisone

Technical Data
Citations (4) / Reviews (1)

Biological Activity for Hydrocortisone

Hydrocortisone is an adrenal glucocorticoid released in the stress response. Increases glucose production by gluconeogenesis. Immunosuppressant and antidiuretic hormone. Used in protocols to generate retinal pigment epithelial (RPE) cells from PSCs.

Compound Libraries for Hydrocortisone

Hydrocortisone is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen FDA-Approved Drugs. Find out more about compound libraries available from Tocris.

Technical Data for Hydrocortisone

M. Wt	362.46
Formula	C₂₁H₃₀O₅
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	50-23-7
PubChem ID	5754
InChi Key	JYGXADMDTFJGBT-VWUMJDOOSA-N
Smiles	O=C1CC[C@@]2(C)C(CC[C@]3([H])[C@@]([H])2[C@@H](O)C[C@@]4(C)[C@]([H])3CC[C@@](O)4[C@@](CO)=O)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Hydrocortisone

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	36.25	100
	ethanol	9.06	25

Preparing Stock Solutions for Hydrocortisone

The following data is based on the product molecular weight 362.46.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.76 mL	13.79 mL	27.59 mL
5 mM	0.55 mL	2.76 mL	5.52 mL
10 mM	0.28 mL	1.38 mL	2.76 mL
50 mM	0.06 mL	0.28 mL	0.55 mL

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Reconstitution Calculator

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References for Hydrocortisone

References are publications that support the biological activity of the product.

Surendran An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells STAR Protoc. 2022 PMID: 36386870
Khani and Tayek Cortisol increases gluconeogenesis in humans; its role in the metabolic syndrome. Clin.Sci.(Lond) 2001 PMID: 11724664

Citations for Hydrocortisone (4)

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Citations are publications that use Bio-Techne products. Selected citations for Hydrocortisone include:

Georges et al. (2022) Polyploid giant cancer cells, EZH2 and Myc upregulation in mammary epithelial cells infected with high-risk human cytomegalovirus. EBioMedicine. 80:104056
PMID: 35596973
Paul F et al. (2021) Cell Populations Expressing Stemness-Associated Markers in Lung Adenocarcinoma. Life (Basel). 11
PMID: 34685477
Srinivasan et al. (2018) Androgen receptor expression reduces stemness characteristics of prostate cancer cells (PC3) by repression of CD44 and SOX2. J Cell Biochem. 2018;
PMID: 30206982
Frost et al. (2017) The Use of DQ-BSA to Monitor the Turnover of Autophagy-Associated Cargo. Methods Enzymol. 587:43
PMID: 28253971

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Customer Reviews for Hydrocortisone (1)

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cell culture

Name: Irving Garcia

Species: Human

Assay Type: In Vitro

Cell Line/Tissue: ES cells

Used before always use from Tocris. Always works well in cell culture.

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