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StemXVivo Osteogenic/Adipogenic Base Media Best Seller
Catalog # CCM007 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Detection of Osteocalcin in Human MSCs-differentiated Osteocytes. Human MSCs were differentiatedin vitrofor 14 days using Human/Mouse/Rat StemXVivo®Osteogenic/Adipogenic Base Media (Catalog #CCM007) and Human StemXVivo®Osteogenic Supplement (Catalog # CCM008). Osteocyte differentiation was verified using a Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB1419). The cells were stained with a NorthernLights™(NL)557-conjugated Donkey Anti-Mouse Secondary Antibody (Catalog # NL007; red), and the nuclei were counterstained with DAPI (blue). |
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Detection of Osteopontin in Mouse MSC-differentiated Osteocytes. Mouse MSCs were cultured for 14 days using the Human/Mouse/Rat StemXVivo®Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Mouse/Rat StemXVivo®Osteogenic Supplement (Catalog # CCM009). Osteocyte differentiation was verified using a Goat Anti-Mouse Osteopontin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The cells were stained using a NorthernLights™557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # NL001; red) and the nuclei were counterstained with DAPI (blue). |
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Detection of Osteocalcin in Rat MSC-differentiated Osteocytes. Rat MSCs were cultured for 14 days using the Human/Mouse/Rat StemXVivo®Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Mouse/Rat StemXVivo®Osteogenic Supplement (Catalog # CCM009). Osteocyte differentiation was verified using a Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB1419). The cells were stained using a NorthernLights™557-conjugated Donkey Anti-Mouse Secondary Antibody (Catalog # NL007; red) and the nuclei were counterstained with DAPI (blue). |
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Detection of FABP4 in Human MSC-differentiated Adipocytes. Human MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo®Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Human/Mouse/Rat StemXVivo®Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipocytes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443). The cells were stained with a NorthernLights™557-conjugated Donkey Anti-Mouse Secondary Antibody (Catalog # NL001; red) and the nuclei were counterstained with DAPI (blue). |
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Detection of FABP4 in Mouse MSC-differentiated Adipocytes Mouse MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo®Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Human/Mouse/Rat StemXVivo®Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipocytes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443). The cells were stained with a NorthernLights™557-conjugated Donkey Anti-Mouse Secondary Antibody (Catalog # NL007; red) and the nuclei were counterstained with DAPI (blue). |
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Detection of FABP4 in Rat MSC-differentiated Adipocytes. Rat MSCs were differentiated for 14 days using the Human/Mouse/Rat StemXVivo®Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Human/Mouse/Rat StemXVivo®Adipogenic Supplement (Catalog # CCM011). Mature differentiated adipocytes were detected with a Goat Anti-Mouse FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443). The cells were stained with a NorthernLights™557-conjugated Donkey Anti-Mouse Secondary Antibody (Catalog # NL007; red) and the nuclei were counterstained with DAPI (blue). |
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, human, mouse, or rat MSCs are differentiated into adipocytes or osteocytes using the following in vitro differentiation procedure:
- Culture multipotent cells of interest
- Induce adipogenic or osteogenic differentiation using media supplements
- Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC
For use with Human StemXVivo® Osteogenic Supplement (Catalog # CCM008), Mouse/Rat Osteogenic Supplement (Catalog # CCM009), or Human/Mouse/Rat StemXVivo® Adipogenic Supplement (Catalog # CCM011).
Reagents Provided
Reagents supplied in the Human/Mouse/Rat StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007):
- 250 mL of StemXVivo® Osteogenic/Adipogenic Base Media
Other Supplies Required
Reagents
- Human StemXVivo® Osteogenic Supplement (Catalog # CCM008) or Mouse/Rat Osteogenic Supplement (Catalog # CCM009)
- Human/Mouse/Rat StemXVivo® Adipogenic Supplement (Catalog # CCM011)
- Penicillin-Streptomycin-Glutamate (100X)
Materials
- MSCs
- 10 cm tissue culture plates
- 15 mL centrifuge tubes
- Pipettes and pipette tips
- Serological pipettes
Equipment
- 37 °C and 5% CO2 incubator
- Centrifuge
- Hemocytometer
- Inverted microscope
- 2 °C to 8 °C refrigerator
- 37 °C water bath
Procedure Overview
This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.
Osteogenic Differentiation
Plate 4.2 x 103 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.
Culture cells to 50-70% confluency.
Replace the medium with StemXVivo® Osteogenic Differentiation Medium to induce osteogenesis.
Every 3-4 days, replace with fresh Osteogenic Differentiation Medium.
After 14-21 days, osteocytes can be harvested and analyzed.
Adipogenic Differentiation
Plate 2.1 x 104 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.
Culture cells to 100% confluency.
Replace the medium in with Adipogenic Differentiation Medium to induce adipogenesis.
Every 3-4 days, replace with fresh Adipogenic Differentiation Medium.
After 14-21 days, adipocytes can be harvested and analyzed.
Citations for StemXVivo Osteogenic/Adipogenic Base Media (36)
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Citations are publications that use Bio-Techne products. Selected citations for StemXVivo Osteogenic/Adipogenic Base Media include:
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Species: Canine
Sample Types: Whole Cells
RF Scassiotti et al. (2022-12-13), Adipose and amnion-derived mesenchymal stem cells: Extracellular vesicles characterization and implication for reproductive biotechnology Theriogenology, 2022-1198(0):264-272.
PMID: 36623429 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayK Jabbari et al. (2022-10-22), CD36+ Fibroblasts Secrete Protein Ligands That Growth-Suppress Triple-Negative Breast Cancer Cells While Elevating Adipogenic Markers for a Model of Cancer-Associated Fibroblast International Journal of Molecular Sciences, 2022-123(21).
PMID: 36361532 -
Species: Human
Sample Types: Whole Cell
Applications: Cell CultureCH Bucher et al. (2022-08-26), Local immune cell contributions to fracture healing in aged individuals - A novel role for interleukin 22 Experimental Molecular Medicine, 2022-08-26;54(8):1262-1276.
PMID: 36028760 -
Species: Human
Sample Types: Whole Cells
SI Hodgetts et al. (2022-06-01), Effects of amyloid precursor protein peptide APP96-110, alone or with human mesenchymal stromal cells, on recovery after spinal cord injury Neural regeneration research, 2022-017(6):1376-1386.
PMID: 34782585 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayKK Sivaraj et al. (2021-07-13), Regional specialization and fate specification of bone stromal cells in skeletal development Cell Reports, 2021-036(2):109352.
PMID: 34260921 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayN Jeger-Madi et al. (2021-04-16), Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation Scientific Reports, 2021-011(1):8355.
PMID: 33863936 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Thurner et al. (2020-06-12), Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration Stem Cell Res Ther, 2020-011(1):233.
PMID: 32532320 -
S Jeong et al. (2020-01-20), BMP4 and perivascular cells promote hematopoietic differentiation of human pluripotent stem cells in a differentiation stage-specific manner Exp. Mol. Med., 2020-00(0).
PMID: 31956269 -
Species: Equine
Sample Types: Whole Cells
L Bourebaba et al. (2019-12-17), Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome Stem Cell Res Ther, 2019-110(1):392.
PMID: 31847882 -
Species: Human
Sample Types: Whole Cells
S Khetan et al. (2019-08-02), Maintenance of stem cell viability and differentiation potential following cryopreservation within 3-dimensional hyaluronic acid hydrogels Cryobiology, 2019-00(0).
PMID: 31381877 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayS Wasnik et al. (2018-09-06), 1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites JCI Insight, 2018-03(17).
PMID: 30185660 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayC Tang et al. (2018-08-29), Chemotherapy-induced niche perturbs hematopoietic reconstitution in B-cell acute lymphoblastic leukemia J. Exp. Clin. Cancer Res., 2018-037(1):204.
PMID: 30157922 -
Species: Human
Sample Types: Whole Cells
A Trillhaase et al. (2018-07-17), Differentiation of human iPSCs into VSMCs and generation of VSMC-derived calcifying vascular cells Stem Cell Res, 2018-031(0):62-70.
PMID: 30029055 -
Species: Human
Sample Types: Whole Cells
A Galarza To et al. (2018-06-12), An immortalised mesenchymal stem cell line maintains mechano-responsive behaviour and can be used as a reporter of substrate stiffness Sci Rep, 2018-08(1):8981.
PMID: 29895825 -
Species: Equine
Sample Types: Whole Cells
Applications: BioassayK Marycz et al. (2018-06-06), Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses Stem Cells Int, 2018-02018(0):5340756.
PMID: 29977307 -
Species: Mouse
Sample Types: Whole Cells
C Liu et al. (2018-05-08), Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-? Stem Cells Int, 2018-02018(0):4069032.
PMID: 29853911 -
Species: Porcine
Sample Types: Whole Cells
Applications: BioassayS La Frances et al. (2018-03-07), Long-term regeneration and remodeling of the pig esophagus after circumferential resection using a retrievable synthetic scaffold carrying autologous cells Sci Rep, 2018-08(1):4123.
PMID: 29515136 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMH Abumaree et al. (2017-05-04), Human chorionic villous mesenchymal stem/stromal cells modify the effects of oxidative stress on endothelial cell functions Placenta, 2017-00(0).
PMID: 28502524 -
Species: Human
Sample Types: Whole Cells
HR Lin et al. (2017-01-10), Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods Sci Rep, 2017-07(0):40069.
PMID: 28071738 -
Species: Mouse
Sample Types: Whole Cells
(2016-12-19), Enhancement of Adipocyte Browning by Angiotensin II Type 1 Receptor Blockade PLoS ONE, 2016-111(12):e0167704.
PMID: 27992452 -
Species: Equine
Sample Types: Whole Cells
K Marycz et al. (2016-12-08), Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation Oxid Med Cell Longev, 2016-12016(0):3718468.
PMID: 28053691 -
Kenneth R Chien (2016-11-01), An IGF1R-Dependent Pathway Drives Epicardial Adipose Tissue Formation After Myocardial Injury Circulation, 2016-10(0).
PMID: 27803039 -
Species: Feline
Sample Types: Whole Cells
Applications: Cell CultureVidane A et al. (2014-08-27), Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation. Stem Cells Cloning, 2014-07(0):71-8.
PMID: 25249758 -
Species: Mouse
Sample Types: Whole Cells
Ishikawa et al. (2014-08-18), MCP/CCR2 signaling is essential for recruitment of mesenchymal progenitor cells during the early phase of fracture healing. PLoS ONE, 2014-09(8):e104954.
PMID: 25133509 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayChen D et al. (2014-02-22), Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes. Biomaterials, 2014-035(14):4278-87.
PMID: 24565521 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayDriskell R et al. (2013-12-12), Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013-1504(7479):277-81.
PMID: 24336287 -
Species: Human
Sample Types: Whole Cells
Osada M et al. (2013-12-10), Label retention identifies a multipotent mesenchymal stem cell-like population in the postnatal thymus. PLoS ONE, 2013-18(12):e83024.
PMID: 24340075 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureKao T et al. (2013-09-11), Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments. J Biomed Mater Res B Appl Biomater, 2013-0102(3):463-76.
PMID: 24039170 -
Species: Mouse
Sample Types: Whole Cells
Applications: Cell CultureQian H et al. (2012-11-26), Molecular characterization of prospectively isolated multipotent mesenchymal progenitors provides new insight into the cellular identity of mesenchymal stem cells in mouse bone marrow. Mol Cell Biol, 2012-133(4):661-77.
PMID: 23184664 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGerlach J et al. (2012-10-16), Perivascular mesenchymal progenitors in human fetal and adult liver. Stem Cells Dev, 2012-121(18):3258-69.
PMID: 22931482 -
Species: Human
Sample Types: Whole Cells
Yu X et al. (2012-05-01), miR-125b Is an adhesion-regulated microRNA that protects mesenchymal stem cells from anoikis. Stem Cells, 2012-030(5):956-64.
PMID: 22331826 -
Species: Mouse
Sample Types: Whole Cells
Saito S et al. (2011-07-08), Mesenchymal stem cells stably transduced with a dominant-negative inhibitor of CCL2 greatly attenuate bleomycin-induced lung damage. Am. J. Pathol., 2011-0179(3):1088-94.
PMID: 21741938 -
Species: Mouse
Sample Types: Whole Cells
Hoffman AM et al. (2011-07-06), Lung-derived mesenchymal stromal cell post-transplantation survival, persistence, paracrine expression, and repair of elastase-injured lung. Stem Cells Dev., 2011-020(10):1779-92.
PMID: 21585237 -
Species: Human
Sample Types: Whole Cells
Giuliani M et al. (2011-05-19), Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation. 2011-06(5):e19988.
PMID: 21625521 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNagai K et al. (2010-04-07), Long-term culture following ES-like gene-induced reprogramming elicits an aggressive phenotype in mutated cholangiocellular carcinoma cells. Biochem. Biophys. Res. Commun., 2010-0395(2):258-63.
PMID: 20381452 -
Species: Human
Sample Types: Whole Cells
Wang W et al. (2009-02-12), 3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells. Biomaterials, 2009-030(14):2705-15.
PMID: 19215979
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