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StemXVivo Osteogenic/Adipogenic Base Media

Key Product Details

Detection of Osteocalcin in Human MSCs-differentiated Osteocytes. 
(7)
Detection of Osteopontin in Mouse MSC-differentiated Osteocytes.
Detection of Osteocalcin in Rat MSC-differentiated Osteocytes. 
Detection of FABP4 in Human MSC-differentiated Adipocytes.
Detection of FABP4 in Mouse MSC-differentiated Adipocytes
Detection of FABP4 in Rat MSC-differentiated Adipocytes.
StemXVivo Osteogenic/Adipogenic Base Media
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CCM007

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, human, mouse, or rat MSCs are differentiated into adipocytes or osteocytes using the following in vitro differentiation procedure:

  • Culture multipotent cells of interest
  • Induce adipogenic or osteogenic differentiation using media supplements
  • Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC

For use with Human StemXVivo® Osteogenic Supplement (Catalog # CCM008), Mouse/Rat Osteogenic Supplement (Catalog # CCM009), or Human/Mouse/Rat StemXVivo® Adipogenic Supplement (Catalog # CCM011).

 

 

Reagents Provided

Reagents supplied in the Human/Mouse/Rat StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007):

  • 250 mL of StemXVivo® Osteogenic/Adipogenic Base Media

 

Other Supplies Required

Reagents

  • Human StemXVivo® Osteogenic Supplement (Catalog # CCM008) or Mouse/Rat Osteogenic Supplement (Catalog # CCM009)
  • Human/Mouse/Rat StemXVivo® Adipogenic Supplement (Catalog # CCM011)
  • Penicillin-Streptomycin-Glutamate (100X)

Materials

  • MSCs
  • 10 cm tissue culture plates
  • 15 mL centrifuge tubes
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.

Osteogenic Differentiation

Plate 4.2 x 103 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.

Culture cells to 50-70% confluency.

Osteogenic Differentiation

Replace the medium with StemXVivo® Osteogenic Differentiation Medium to induce osteogenesis.

Osteogenic Differentiation

Every 3-4 days, replace with fresh Osteogenic Differentiation Medium.

 

After 14-21 days, osteocytes can be harvested and analyzed.

Osteogenic Differentiation

Adipogenic Differentiation

 

Plate 2.1 x 104 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.

Culture cells to 100% confluency.

Subculturing Mesenchymal Stem Cells

Replace the medium in with Adipogenic Differentiation Medium to induce adipogenesis.

Subculturing Mesenchymal Stem Cells

Every 3-4 days, replace with fresh Adipogenic Differentiation Medium.

 

After 14-21 days, adipocytes can be harvested and analyzed.

Subculturing Mesenchymal Stem Cells

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  • StemXVivo Osteogenic/Adipogenic Base Media
    Name: Anonymous
    Verified Customer | Posted 01/18/2019
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    Name: Anonymous
    Verified Customer | Posted 04/04/2018

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