StemXVivo Osteogenic/Adipogenic Base Media Best Seller
Catalog # CCM007 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, human, mouse, or rat MSCs are differentiated into adipocytes or osteocytes using the following in vitro differentiation procedure:
- Culture multipotent cells of interest
- Induce adipogenic or osteogenic differentiation using media supplements
- Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC
For use with Human StemXVivo® Osteogenic Supplement (Catalog # CCM008), Mouse/Rat Osteogenic Supplement (Catalog # CCM009), or Human/Mouse/Rat StemXVivo® Adipogenic Supplement (Catalog # CCM011).
Reagents supplied in the Human/Mouse/Rat StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007):
- 250 mL of StemXVivo® Osteogenic/Adipogenic Base Media
Reagents
- Human StemXVivo® Osteogenic Supplement (Catalog # CCM008) or Mouse/Rat Osteogenic Supplement (Catalog # CCM009)
- Human/Mouse/Rat StemXVivo® Adipogenic Supplement (Catalog # CCM011)
- Penicillin-Streptomycin-Glutamate (100X)
Materials
- MSCs
- 10 cm tissue culture plates
- 15 mL centrifuge tubes
- Pipettes and pipette tips
- Serological pipettes
Equipment
- 37 °C and 5% CO2 incubator
- Centrifuge
- Hemocytometer
- Inverted microscope
- 2 °C to 8 °C refrigerator
- 37 °C water bath
This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.
Osteogenic Differentiation
Plate 4.2 x 103 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.
Culture cells to 50-70% confluency.

Replace the medium with StemXVivo® Osteogenic Differentiation Medium to induce osteogenesis.

Every 3-4 days, replace with fresh Osteogenic Differentiation Medium.
After 14-21 days, osteocytes can be harvested and analyzed.

Adipogenic Differentiation
Plate 2.1 x 104 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.
Culture cells to 100% confluency.

Replace the medium in with Adipogenic Differentiation Medium to induce adipogenesis.

Every 3-4 days, replace with fresh Adipogenic Differentiation Medium.
After 14-21 days, adipocytes can be harvested and analyzed.

Citations for StemXVivo Osteogenic/Adipogenic Base Media (36)
Citations are publications that use Bio-Techne products. Selected citations for StemXVivo Osteogenic/Adipogenic Base Media include:
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Species: Canine
Sample Types: Whole Cells
RF Scassiotti et al. (2022-12-13), Adipose and amnion-derived mesenchymal stem cells: Extracellular vesicles characterization and implication for reproductive biotechnology Theriogenology, 2022-1198(0):264-272.
PMID: 36623429 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayK Jabbari et al. (2022-10-22), CD36+ Fibroblasts Secrete Protein Ligands That Growth-Suppress Triple-Negative Breast Cancer Cells While Elevating Adipogenic Markers for a Model of Cancer-Associated Fibroblast International Journal of Molecular Sciences, 2022-123(21).
PMID: 36361532 -
Species: Human
Sample Types: Whole Cell
Applications: Cell CultureCH Bucher et al. (2022-08-26), Local immune cell contributions to fracture healing in aged individuals - A novel role for interleukin 22 Experimental Molecular Medicine, 2022-08-26;54(8):1262-1276.
PMID: 36028760 -
Species: Human
Sample Types: Whole Cells
SI Hodgetts et al. (2022-06-01), Effects of amyloid precursor protein peptide APP96-110, alone or with human mesenchymal stromal cells, on recovery after spinal cord injury Neural regeneration research, 2022-017(6):1376-1386.
PMID: 34782585 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayKK Sivaraj et al. (2021-07-13), Regional specialization and fate specification of bone stromal cells in skeletal development Cell Reports, 2021-036(2):109352.
PMID: 34260921 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayN Jeger-Madi et al. (2021-04-16), Self-organization and culture of Mesenchymal Stem Cell spheroids in acoustic levitation Scientific Reports, 2021-011(1):8355.
PMID: 33863936 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Thurner et al. (2020-06-12), Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration Stem Cell Res Ther, 2020-011(1):233.
PMID: 32532320 -
S Jeong et al. (2020-01-20), BMP4 and perivascular cells promote hematopoietic differentiation of human pluripotent stem cells in a differentiation stage-specific manner Exp. Mol. Med., 2020-00(0).
PMID: 31956269 -
Species: Equine
Sample Types: Whole Cells
L Bourebaba et al. (2019-12-17), Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose-derived mesenchymal stromal stem cells affected by metabolic syndrome Stem Cell Res Ther, 2019-110(1):392.
PMID: 31847882 -
Species: Human
Sample Types: Whole Cells
S Khetan et al. (2019-08-02), Maintenance of stem cell viability and differentiation potential following cryopreservation within 3-dimensional hyaluronic acid hydrogels Cryobiology, 2019-00(0).
PMID: 31381877 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayS Wasnik et al. (2018-09-06), 1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites JCI Insight, 2018-03(17).
PMID: 30185660 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayC Tang et al. (2018-08-29), Chemotherapy-induced niche perturbs hematopoietic reconstitution in B-cell acute lymphoblastic leukemia J. Exp. Clin. Cancer Res., 2018-037(1):204.
PMID: 30157922 -
Species: Human
Sample Types: Whole Cells
A Trillhaase et al. (2018-07-17), Differentiation of human iPSCs into VSMCs and generation of VSMC-derived calcifying vascular cells Stem Cell Res, 2018-031(0):62-70.
PMID: 30029055 -
Species: Human
Sample Types: Whole Cells
A Galarza To et al. (2018-06-12), An immortalised mesenchymal stem cell line maintains mechano-responsive behaviour and can be used as a reporter of substrate stiffness Sci Rep, 2018-08(1):8981.
PMID: 29895825 -
Species: Equine
Sample Types: Whole Cells
Applications: BioassayK Marycz et al. (2018-06-06), Evaluation of Oxidative Stress and Mitophagy during Adipogenic Differentiation of Adipose-Derived Stem Cells Isolated from Equine Metabolic Syndrome (EMS) Horses Stem Cells Int, 2018-02018(0):5340756.
PMID: 29977307 -
Species: Mouse
Sample Types: Whole Cells
C Liu et al. (2018-05-08), Mesenchymal Stem Cells Promote the Osteogenesis in Collagen-Induced Arthritic Mice through the Inhibition of TNF-? Stem Cells Int, 2018-02018(0):4069032.
PMID: 29853911 -
Species: Porcine
Sample Types: Whole Cells
Applications: BioassayS La Frances et al. (2018-03-07), Long-term regeneration and remodeling of the pig esophagus after circumferential resection using a retrievable synthetic scaffold carrying autologous cells Sci Rep, 2018-08(1):4123.
PMID: 29515136 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMH Abumaree et al. (2017-05-04), Human chorionic villous mesenchymal stem/stromal cells modify the effects of oxidative stress on endothelial cell functions Placenta, 2017-00(0).
PMID: 28502524 -
Species: Human
Sample Types: Whole Cells
HR Lin et al. (2017-01-10), Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods Sci Rep, 2017-07(0):40069.
PMID: 28071738 -
Species: Mouse
Sample Types: Whole Cells
(2016-12-19), Enhancement of Adipocyte Browning by Angiotensin II Type 1 Receptor Blockade PLoS ONE, 2016-111(12):e0167704.
PMID: 27992452 -
Species: Equine
Sample Types: Whole Cells
K Marycz et al. (2016-12-08), Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation Oxid Med Cell Longev, 2016-12016(0):3718468.
PMID: 28053691 -
Kenneth R Chien (2016-11-01), An IGF1R-Dependent Pathway Drives Epicardial Adipose Tissue Formation After Myocardial Injury Circulation, 2016-10(0).
PMID: 27803039 -
Species: Feline
Sample Types: Whole Cells
Applications: Cell CultureVidane A et al. (2014-08-27), Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation. Stem Cells Cloning, 2014-07(0):71-8.
PMID: 25249758 -
Species: Mouse
Sample Types: Whole Cells
Ishikawa et al. (2014-08-18), MCP/CCR2 signaling is essential for recruitment of mesenchymal progenitor cells during the early phase of fracture healing. PLoS ONE, 2014-09(8):e104954.
PMID: 25133509 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayChen D et al. (2014-02-22), Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes. Biomaterials, 2014-035(14):4278-87.
PMID: 24565521 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayDriskell R et al. (2013-12-12), Distinct fibroblast lineages determine dermal architecture in skin development and repair. Nature, 2013-1504(7479):277-81.
PMID: 24336287 -
Species: Human
Sample Types: Whole Cells
Osada M et al. (2013-12-10), Label retention identifies a multipotent mesenchymal stem cell-like population in the postnatal thymus. PLoS ONE, 2013-18(12):e83024.
PMID: 24340075 -
Species: Human
Sample Types: Whole Cells
Applications: Cell CultureKao T et al. (2013-09-11), Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments. J Biomed Mater Res B Appl Biomater, 2013-0102(3):463-76.
PMID: 24039170 -
Species: Mouse
Sample Types: Whole Cells
Applications: Cell CultureQian H et al. (2012-11-26), Molecular characterization of prospectively isolated multipotent mesenchymal progenitors provides new insight into the cellular identity of mesenchymal stem cells in mouse bone marrow. Mol Cell Biol, 2012-133(4):661-77.
PMID: 23184664 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGerlach J et al. (2012-10-16), Perivascular mesenchymal progenitors in human fetal and adult liver. Stem Cells Dev, 2012-121(18):3258-69.
PMID: 22931482 -
Species: Human
Sample Types: Whole Cells
Yu X et al. (2012-05-01), miR-125b Is an adhesion-regulated microRNA that protects mesenchymal stem cells from anoikis. Stem Cells, 2012-030(5):956-64.
PMID: 22331826 -
Species: Mouse
Sample Types: Whole Cells
Saito S et al. (2011-07-08), Mesenchymal stem cells stably transduced with a dominant-negative inhibitor of CCL2 greatly attenuate bleomycin-induced lung damage. Am. J. Pathol., 2011-0179(3):1088-94.
PMID: 21741938 -
Species: Mouse
Sample Types: Whole Cells
Hoffman AM et al. (2011-07-06), Lung-derived mesenchymal stromal cell post-transplantation survival, persistence, paracrine expression, and repair of elastase-injured lung. Stem Cells Dev., 2011-020(10):1779-92.
PMID: 21585237 -
Species: Human
Sample Types: Whole Cells
Giuliani M et al. (2011-05-19), Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation. 2011-06(5):e19988.
PMID: 21625521 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNagai K et al. (2010-04-07), Long-term culture following ES-like gene-induced reprogramming elicits an aggressive phenotype in mutated cholangiocellular carcinoma cells. Biochem. Biophys. Res. Commun., 2010-0395(2):258-63.
PMID: 20381452 -
Species: Human
Sample Types: Whole Cells
Wang W et al. (2009-02-12), 3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells. Biomaterials, 2009-030(14):2705-15.
PMID: 19215979
There are no citations that match your criteria.