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StemXVivo Human Osteogenic Supplement, 12.5 mL

Catalog # CCM008 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Detection of Osteocalcin in Human MSC-differentiated Osteocytes.
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Setting the standard in quality research reagents

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, human MSCs are differentiated into osteocytes using the following in vitro differentiation procedure:

  • Culture multipotent cells of interest
  • Induce osteogenic differentiation using a media supplement
  • Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC

For use with Human/Mouse/Rat StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007).

 

 

Reagents Provided
plied in the Human/

Reagents supplied in the Human StemXVivo® Osteogenic Supplement (Catalog # CCM008):

  • 12.5 mL of StemXVivo® Osteogenic Supplement

    Note: The quantity of osteogenic media supplement is sufficient to make 250 mL of media for differentiation.

 

Other Supplies Required

Reagents

  • StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007 or equivalent)
  • Penicillin-Streptomycin-Glutamate (100X)

Materials

  • Human MSCs
  • 10 cm tissue culture plates
  • 15 mL centrifuge tubes
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.

Osteogenic Differentiation

Plate 4.2 x 103 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.

Culture cells to 50-70% confluency.

Protocol for Human CD4+ T Cell Enrichment Column

Replace the medium with StemXVivo® Osteogenic Differentiation Medium to induce osteogenesis.

Protocol for Human CD4+ T Cell Enrichment Column

Every 3-4 days, replace with fresh Osteogenic Differentiation Medium.

 

After 14 days, osteocytes can be harvested and analyzed.

Protocol for Human CD4+ T Cell Enrichment Column

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  • StemXVivo Human Osteogenic Supplement, 12.5 mL
    Name: Abby Sukarto
    Verified Customer | Posted 01/18/2019

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FAQs for StemXVivo Human Osteogenic Supplement, 12.5 mL

  • In the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006), are Part #'s 90415, 390416, and 390417 the same as the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo® Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006), respectively?

    Yes, the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo®  Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006) are the same as Part #'s 390415, 390416, and 390417, respectively, in the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006).

Product Documents for StemXVivo Human Osteogenic Supplement, 12.5 mL

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.