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StemXVivo Human Osteogenic Supplement, 12.5 mL

Detection of Osteocalcin in Human MSC-differentiated Osteocytes.
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CCM008
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, human MSCs are differentiated into osteocytes using the following in vitro differentiation procedure:

  • Culture multipotent cells of interest
  • Induce osteogenic differentiation using a media supplement
  • Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC

For use with Human/Mouse/Rat StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007).

 

 

Reagents Provided
plied in the Human/

Reagents supplied in the Human StemXVivo® Osteogenic Supplement (Catalog # CCM008):

  • 12.5 mL of StemXVivo® Osteogenic Supplement

    Note: The quantity of osteogenic media supplement is sufficient to make 250 mL of media for differentiation.

 

Other Supplies Required

Reagents

  • StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007 or equivalent)
  • Penicillin-Streptomycin-Glutamate (100X)

Materials

  • Human MSCs
  • 10 cm tissue culture plates
  • 15 mL centrifuge tubes
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.

Osteogenic Differentiation

Plate 4.2 x 103 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.

Culture cells to 50-70% confluency.

Protocol for Human CD4+ T Cell Enrichment Column

Replace the medium with StemXVivo® Osteogenic Differentiation Medium to induce osteogenesis.

Protocol for Human CD4+ T Cell Enrichment Column

Every 3-4 days, replace with fresh Osteogenic Differentiation Medium.

 

After 14 days, osteocytes can be harvested and analyzed.

Protocol for Human CD4+ T Cell Enrichment Column

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    Verified Customer | Posted 01/18/2019

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FAQs

  • In the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006), are Part #'s 90415, 390416, and 390417 the same as the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo® Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006), respectively?

    Yes, the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo®  Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006) are the same as Part #'s 390415, 390416, and 390417, respectively, in the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006).

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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