StemXVivo Human Osteogenic Supplement, 12.5 mL
Catalog # CCM008 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Assay Procedure
Refer to the product datasheet for complete product details.
Briefly, human MSCs are differentiated into osteocytes using the following in vitro differentiation procedure:
- Culture multipotent cells of interest
- Induce osteogenic differentiation using a media supplement
- Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC
For use with Human/Mouse/Rat StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007).
Reagents supplied in the Human StemXVivo® Osteogenic Supplement (Catalog # CCM008):
- 12.5 mL of StemXVivo® Osteogenic Supplement
Note: The quantity of osteogenic media supplement is sufficient to make 250 mL of media for differentiation.
Reagents
- StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007 or equivalent)
- Penicillin-Streptomycin-Glutamate (100X)
Materials
- Human MSCs
- 10 cm tissue culture plates
- 15 mL centrifuge tubes
- Pipettes and pipette tips
- Serological pipettes
Equipment
- 37 °C and 5% CO2 incubator
- Centrifuge
- Hemocytometer
- Inverted microscope
- 2 °C to 8 °C refrigerator
- 37 °C water bath
This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.
Osteogenic Differentiation
Plate 4.2 x 103 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.
Culture cells to 50-70% confluency.

Replace the medium with StemXVivo® Osteogenic Differentiation Medium to induce osteogenesis.

Every 3-4 days, replace with fresh Osteogenic Differentiation Medium.
After 14 days, osteocytes can be harvested and analyzed.

Citations for StemXVivo Human Osteogenic Supplement, 12.5 mL (13)
Citations are publications that use Bio-Techne products. Selected citations for StemXVivo Human Osteogenic Supplement, 12.5 mL include:
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Species: Human
Sample Types: Whole Cell
Applications: Cell CultureCH Bucher et al. (2022-08-26), Local immune cell contributions to fracture healing in aged individuals - A novel role for interleukin 22 Experimental Molecular Medicine, 2022-08-26;54(8):1262-1276.
PMID: 36028760 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayM Thurner et al. (2020-06-12), Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration Stem Cell Res Ther, 2020-011(1):233.
PMID: 32532320 -
S Jeong et al. (2020-01-20), BMP4 and perivascular cells promote hematopoietic differentiation of human pluripotent stem cells in a differentiation stage-specific manner Exp. Mol. Med., 2020-00(0).
PMID: 31956269 -
Species: Human
Sample Types: Whole Cells
A Trillhaase et al. (2018-07-17), Differentiation of human iPSCs into VSMCs and generation of VSMC-derived calcifying vascular cells Stem Cell Res, 2018-031(0):62-70.
PMID: 30029055 -
Species: Human
Sample Types: Whole Cells
A Galarza To et al. (2018-06-12), An immortalised mesenchymal stem cell line maintains mechano-responsive behaviour and can be used as a reporter of substrate stiffness Sci Rep, 2018-08(1):8981.
PMID: 29895825 -
Species: Human
Sample Types: Whole Cells
AM Loye et al. (2018-06-08), Regulation of Mesenchymal Stem Cell Differentiation by Nanopatterning of Bulk Metallic Glass Sci Rep, 2018-08(1):8758.
PMID: 29884812 -
Species: Human
Sample Types: Whole Cells
(2016-01-01), Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling Stem Cells Transl Med, 2016-00(0).
PMID: 27634598 -
Species: Human
Sample Types: Whole Cells
Brady K et al. (2013-12-04), Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis. Stem Cells Dev, 2013-123(5):541-54.
PMID: 24172175 -
Species: Human
Sample Types: Whole Cells
Kao T et al. (2013-09-11), Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments. J Biomed Mater Res B Appl Biomater, 2013-0102(3):463-76.
PMID: 24039170 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGerlach J et al. (2012-10-16), Perivascular mesenchymal progenitors in human fetal and adult liver. Stem Cells Dev, 2012-121(18):3258-69.
PMID: 22931482 -
Species: Human
Sample Types: Whole Cells
Yu X et al. (2012-05-01), miR-125b Is an adhesion-regulated microRNA that protects mesenchymal stem cells from anoikis. Stem Cells, 2012-030(5):956-64.
PMID: 22331826 -
Species: Human
Sample Types: Whole Cells
Giuliani M et al. (2011-05-19), Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation. 2011-06(5):e19988.
PMID: 21625521 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNagai K et al. (2010-04-07), Long-term culture following ES-like gene-induced reprogramming elicits an aggressive phenotype in mutated cholangiocellular carcinoma cells. Biochem. Biophys. Res. Commun., 2010-0395(2):258-63.
PMID: 20381452
There are no citations that match your criteria.
Customer Reviews for StemXVivo Human Osteogenic Supplement, 12.5 mL (1)
FAQs for StemXVivo Human Osteogenic Supplement, 12.5 mL
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In the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006), are Part #'s 90415, 390416, and 390417 the same as the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo® Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006), respectively?
Yes, the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo® Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006) are the same as Part #'s 390415, 390416, and 390417, respectively, in the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006).