In the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006), are Part #'s 90415, 390416, and 390417 the same as the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo® Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006), respectively?

Name: StemXVivo Human Osteogenic Supplement, 12.5 mL
Brand: R&D Systems
SKU: CCM008
Rating: 5 (1 reviews)

Yes, the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo® Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006) are the same as Part #'s 390415, 390416, and 390417, respectively, in the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006).

StemXVivo Human Osteogenic Supplement, 12.5 mL

Catalog # CCM008 | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (13)

Product Datasheet / COA / SDS

Key Product Details

Setting the standard in quality research reagents

Summary for StemXVivo Human Osteogenic Supplement, 12.5 mL

Kit Summary

Media supplement to induce the differentiation of human MSCs into osteocytes. For use with Human/Mouse/Rat StemXVivo Osteogenic/Adipogenic Base Media (Catalog # CCM007).

Key Benefits

Supports induction of osteogenesis in human MSCs
Defined supplement to reduce experimental variation
Developed and optimized using human MSCs

Why Induce Osteogenesis in MSCs with a Defined Media Supplement?

Despite the well-characterized factors and protocols used to differentiate mesenchymal stem/stromal cells (MSCs) into osteocytes, differentiation efficiencies can vary depending on the quality of the MSC starting population and the reagents used to expand and differentiate MSCs.

StemXVivo^® Osteogenic Supplement:

Contains high quality differentiation factors to drive reproducible and efficient MSC osteogenesis.
Is defined to reduce unwanted experimental variability.
Has been developed and optimized using MSCs.

Mesenchymal Stromal Cells or Mesenchymal Stem Cells?

The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’

Mesenchymal stem cells are multipotent, self-renewing cells that have the ability to differentiate into adipocytes, chondrocytes, and osteoblasts when cultured in vitro. Read More about MSC Nomenclature

Human StemXVivo^® Osteogenic Supplement Components

This media supplement contains high quality factors to drive human MSC differentiation into osteocytes.

This supplement requires media (not included), such as Human/Mouse/Rat StemXVivo^® Osteogenic/Adipogenic Base Media (Catalog # CCM007) or equivalent.
The quantity of osteogenic media supplement supplied is sufficient to make 250 mL of media for differentiation.

2006 Proposed Change to MSC Nomenclature

Although mesenchymal stromal cells were once referred to as ‘mesenchymal stem cells’, a change to ‘mesenchymal stromal cells’ was proposed by the International Society for Cellular Therapy in 2006.¹

The change in nomenclature originates from two important factors:

Methods used to isolate mesenchymal stem cells yield a heterogeneous population of cells with only a fraction of these cells demonstrating multipotency.
The absence of direct evidence that mesenchymal stem cells can self-renew and differentiate in vivo.

Use of Mesenchymal Stem and Stromal Cell Terminology

Data supporting MSC self-renewal and multipotency have been obtained using in vitro conditions, which does not adequately reflect the in vivo environment. The lack of in vivo data has led some researchers to question the validity of the term ‘mesenchymal stem cell’ providing further support for the use of ‘mesenchymal stromal cells’ to describe MSCs.² While ‘mesenchymal stromal cells’ may be the more scientifically accurate term for MSCs, the two terms are often used interchangeably in the literature. R&D Systems recognizes the use of both mesenchymal stem cells and mesenchymal stromal cells and uses ‘MSC’ to indicate mesenchymal stem/stromal cells to account for both designations.

Definitions of Mesenchymal Stromal Cells and Mesenchymal Stem Cells

Mesenchymal Stromal Cells – A heterogeneous population of cultured cells with similar characteristics such as the ability to adhere to plastic and the expression of specific marker proteins.
Mesenchymal Stem Cells – A subpopulation of mesenchymal stromal cells that have the capacity to self-renew and differentiate into mesodermal lineages when cultured in vitro. The capacity to self-renew and differentiate in vivo has yet to be clearly demonstrated for mesenchymal stem cells.

References

Dominici, M. et al. (2006) Cytotherapy 8:315.
Keating, A. (2012) Cell Stem Cell 10:709.

The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. However, because these biological properties and hierarchical relationships remain to be clearly demonstrated in vivo, the term 'multipotent mesenchymal stromal cells' is often used to distinguish cultured cells from their in vivo precursors. Originally discovered in mouse bone marrow, multipotent mesenchymal stromal cells cultured from a variety of species and tissue types, have been shown to differentiate into progeny of additional lineages including, cardiomyocytes, endothelial cells, hepatocytes, and neural cells. Again, the physiological relevance of these findings remains to be determined.

Product Specifications for StemXVivo Human Osteogenic Supplement, 12.5 mL

Species

Human

Scientific Data Examples for StemXVivo Human Osteogenic Supplement, 12.5 mL

	Detection of Osteocalcin in Human MSC-differentiated Osteocytes. Human MSCs were differentiatedin vitrofor 14 days using Human/Mouse/Rat StemXVivo^®Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Human StemXVivo^®Osteogenic Supplement (Catalog # CCM008). Osteocyte differentiation was verified using a Mouse Anti-Human Osteocalcin Monoclonal Antibody (Catalog # MAB1419). The cells were stained with a NorthernLights^™(NL)557-conjugated Donkey Anti-Mouse Secondary Antibody (Catalog # NL007; red), and the nuclei were counterstained with DAPI (blue).

Preparation & Storage

Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, human MSCs are differentiated into osteocytes using the following in vitro differentiation procedure:

Culture multipotent cells of interest
Induce osteogenic differentiation using a media supplement
Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC

For use with Human/Mouse/Rat StemXVivo^® Osteogenic/Adipogenic Base Media (Catalog # CCM007).

View Graphic Procedure

Reagents Provided

plied in the Human/

Reagents supplied in the Human StemXVivo^® Osteogenic Supplement (Catalog # CCM008):

12.5 mL of StemXVivo^® Osteogenic Supplement
Note: The quantity of osteogenic media supplement is sufficient to make 250 mL of media for differentiation.

Other Supplies Required

Reagents

StemXVivo^® Osteogenic/Adipogenic Base Media (Catalog # CCM007 or equivalent)
Penicillin-Streptomycin-Glutamate (100X)

Materials

Human MSCs
10 cm tissue culture plates
15 mL centrifuge tubes
Pipettes and pipette tips
Serological pipettes

Equipment

37 °C and 5% CO₂ incubator
Centrifuge
Hemocytometer
Inverted microscope
2 °C to 8 °C refrigerator
37 °C water bath

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.

Osteogenic Differentiation

Plate 4.2 x 10³ MSCs/cm² in StemXVivo^® Osteogenic/Adipogenic Base Media.

Culture cells to 50-70% confluency.

Protocol for Human CD4+ T Cell Enrichment Column

Replace the medium with StemXVivo^® Osteogenic Differentiation Medium to induce osteogenesis.

Every 3-4 days, replace with fresh Osteogenic Differentiation Medium.

After 14 days, osteocytes can be harvested and analyzed.

Citations for StemXVivo Human Osteogenic Supplement, 12.5 mL (13)

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Citations are publications that use Bio-Techne products. Selected citations for StemXVivo Human Osteogenic Supplement, 12.5 mL include:

Species: Human
Sample Types: Whole Cell
Applications: Cell Culture

CH Bucher et al. (2022-08-26), Local immune cell contributions to fracture healing in aged individuals - A novel role for interleukin 22 Experimental Molecular Medicine, 2022-08-26;54(8):1262-1276.
PMID: 36028760
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Thurner et al. (2020-06-12), Generation of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration Stem Cell Res Ther, 2020-011(1):233.
PMID: 32532320
S Jeong et al. (2020-01-20), BMP4 and perivascular cells promote hematopoietic differentiation of human pluripotent stem cells in a differentiation stage-specific manner Exp. Mol. Med., 2020-00(0).
PMID: 31956269
Species: Human
Sample Types: Whole Cells

A Trillhaase et al. (2018-07-17), Differentiation of human iPSCs into VSMCs and generation of VSMC-derived calcifying vascular cells Stem Cell Res, 2018-031(0):62-70.
PMID: 30029055
Species: Human
Sample Types: Whole Cells

A Galarza To et al. (2018-06-12), An immortalised mesenchymal stem cell line maintains mechano-responsive behaviour and can be used as a reporter of substrate stiffness Sci Rep, 2018-08(1):8981.
PMID: 29895825
Species: Human
Sample Types: Whole Cells

AM Loye et al. (2018-06-08), Regulation of Mesenchymal Stem Cell Differentiation by Nanopatterning of Bulk Metallic Glass Sci Rep, 2018-08(1):8758.
PMID: 29884812
Species: Human
Sample Types: Whole Cells

(2016-01-01), Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling Stem Cells Transl Med, 2016-00(0).
PMID: 27634598
Species: Human
Sample Types: Whole Cells

Brady K et al. (2013-12-04), Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis. Stem Cells Dev, 2013-123(5):541-54.
PMID: 24172175
Species: Human
Sample Types: Whole Cells

Kao T et al. (2013-09-11), Suppression of cancer-initiating cells and selection of adipose-derived stem cells cultured on biomaterials having specific nanosegments. J Biomed Mater Res B Appl Biomater, 2013-0102(3):463-76.
PMID: 24039170
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gerlach J et al. (2012-10-16), Perivascular mesenchymal progenitors in human fetal and adult liver. Stem Cells Dev, 2012-121(18):3258-69.
PMID: 22931482
Species: Human
Sample Types: Whole Cells

Yu X et al. (2012-05-01), miR-125b Is an adhesion-regulated microRNA that protects mesenchymal stem cells from anoikis. Stem Cells, 2012-030(5):956-64.
PMID: 22331826
Species: Human
Sample Types: Whole Cells

Giuliani M et al. (2011-05-19), Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation. 2011-06(5):e19988.
PMID: 21625521
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Nagai K et al. (2010-04-07), Long-term culture following ES-like gene-induced reprogramming elicits an aggressive phenotype in mutated cholangiocellular carcinoma cells. Biochem. Biophys. Res. Commun., 2010-0395(2):258-63.
PMID: 20381452

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StemXVivo Human Osteogenic Supplement, 12.5 mL

Name: Abby Sukarto

Verified Customer | Posted 01/18/2019

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FAQs for StemXVivo Human Osteogenic Supplement, 12.5 mL

In the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006), are Part #'s 90415, 390416, and 390417 the same as the StemXVivo^® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo^® Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo^® Human Chondrogenic Supplement (Catalog # CCM006), respectively?

Yes, the StemXVivo^® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo^®Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo^®Human Chondrogenic Supplement (Catalog # CCM006) are the same as Part #'s 390415, 390416, and 390417, respectively, in the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006).