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StemXVivo Mouse/Rat Osteogenic Supplement, 12.5 mL

Key Product Details

Detection of Osteopontin in Mouse MSC-differentiated Osteocytes. 
(2)
Detection of Osteocalcin in Rat MSC-differentiated Osteocytes.
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CCM009

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, mouse or rat MSCs are differentiated into osteocytes using the following in vitro differentiation procedure:

  • Culture multipotent cells of interest
  • Induce osteogenic differentiation using a media supplement
  • Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC

For use with Human/Mouse/Rat StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007).

 

 

Reagents Provided

Reagents supplied in the Mouse/Rat StemXVivo® Osteogenic Supplement (Catalog # CCM009):

  • 12.5 mL of StemXVivo® Osteogenic Supplement

    Note: The quantity of osteogenic media supplement supplied is sufficient to make 250 mL of media for differentiation.

 

Other Supplies Required

Reagents

  • StemXVivo® Osteogenic/Adipogenic Base Media (Catalog # CCM007 or equivalent)
  • Penicillin-Streptomycin-Glutamate (100X)

Materials

  • Mouse or rat MSCs
  • 10 cm tissue culture plates
  • 15 mL centrifuge tubes
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.

Osteogenic Differentiation

Plate 4.2 x 103 MSCs/cm2 in StemXVivo® Osteogenic/Adipogenic Base Media.

Culture cells to 50-70% confluency.

Culture cells to 50-70% confluency.

Replace the medium with StemXVivo® Osteogenic Differentiation Medium to induce osteogenesis.

Replace the medium with StemXVivo  Osteogenic Differentiation Medium to induce osteogenesis.

Every 3-4 days, replace with fresh Osteogenic Differentiation Medium.

 

After 14 days, osteocytes can be harvested and analyzed.

After 14 days, osteocytes can be harvested and analyzed.

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