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StemXVivo Human/Mouse Chondrogenic Supplement, 0.5 mL

Detection of Aggrecan in a Human MSC-differentiated Chondrogenic Pellet Section.
(3)
MSCs Differentiated into Chondrocytes form Characteristic Cell Pellets. 
Detection of Collagen II in a Mouse MSC-differentiated Chondrogenic Pellet Section.
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CCM006
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Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, human or mouse MSCs are differentiated into chondrocytes using the following in vitro differentiation procedure:

  • Culture multipotent cells of interest
  • Induce chondrogenic differentiation using a media supplement
  • Evaluate differentiation using a mature phenotype marker antibody and fluorescent ICC

For use with Human/Mouse/Rat StemXVivo® Chondrogenic Base Media (Catalog # CCM005).

 

 

Reagents Provided

Reagents supplied in the Human/Mouse StemXVivo® Chondrogenic Supplement (Catalog # CCM006):

  • 0.5 mL of StemXVivo® Chondrogenic Supplement

 

Other Supplies Required

Reagents

  • Human/Mouse/Rat StemXVivo® Chondrogenic Base Media (Catalog # CCM005)
  • Penicillin-Streptomycin-Glutamate (100X)

Materials

  • MSCs
  • 15 mL centrifuge tubes
  • Pipettes and pipette tips
  • Serological pipettes

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be optimized.

Transfer 2.5 x 105 MSCs to a 15 mL conical tube.

Centrifuge and resuspend the cells in Chondrogenic Differentiation Medium.

Protocol for Human CD4+ T Cell Enrichment Column

Centrifuge the cells but do not remove the medium.

Every 2-3 days, replace with fresh Chondrogenic Differentiation Medium.

After 14-21 days, the chondrogenic pellet can be harvested and analyzed.

Protocol for Human CD4+ T Cell Enrichment Column

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FAQs

  • In the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006), are Part #'s 90415, 390416, and 390417 the same as the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo® Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006), respectively?

    Yes, the StemXVivo® Human Adipogenic Supplement (Catalog # CCM011), StemXVivo®  Human Osteogenic Supplement (Catalog # CCM008), and StemXVivo® Human Chondrogenic Supplement (Catalog # CCM006) are the same as Part #'s 390415, 390416, and 390417, respectively, in the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006).

  • Are there any experimental tips/hints for successful chondrogenic differentiation of mesenchymal stem cells?

    The following tips/hints are useful for chondrogenic differentiation:a) The mesenchymal stem cells (MSCs) should not be from a late passage (passage 8 or less), b) if using the Human Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC006) or the StemXVivo® Chondrogenic Supplement (Catalog # CCM006), use the starting MSC cell number that is indicated in the protocol, c) Early during chondrogenic differentiation a pellet should form. As differentiation progresses, the pellet will grow and take up a ball-like appearance. d) The pellet should not attach to the tube, therefore care should be taken to not dislodge it while changing media.

Product Datasheets

Certificate of Analysis

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