GMP Human T Cell Media

T Cells Cultured in GMP Human T Cell Media are Viable and Undergo Robust Expansion.  Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 10 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and 5% GMP human AB serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for viability (A), cell density (B), and fold expansion (C). Data is the average of 5 T cell donors with error bars ± SD.

GMP Human T Cell Media Supports T Cell Editing With Lentivirus. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 10 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and 5% GMP human AB serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for expression of the inserted cargo CD19-CAR by recombinant fluorokine (Catalog # ATM9269) and eGFP by flow cytometry. Data is the average of 5 T cell donors with error bars ± SD.

GMP Human T Cell Media Supports Generation of T_SCM and T_CM cells. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 10 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and 5% GMP human AB serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express CD19-CAR and eGFP. After 8 total days in culture, cells were assessed for frequency of CD4+ or CD8+ T cells (A), CD4+ (B), and CD8+ memory cell subsets (C). T cell memory subsets are gated on CD45RA and CCR7, as stem cell memory (T_SCM ,CD45RA+ CCR7+), central memory (T_CM, CD45RA- CCR7+), effector memory (T_EM, CD45RA- CCR7-), and effector (T_eff, CD45RA+ CCR7-). Data is the average of 5 T cell donors with error bars ± SD.

T Cells Express Low Levels of PD-1 After Culture in GMP Human T Cell Media. Purified CD4+ and CD8+ T cells were cultured in a 6M G-Rex® Bioreactor in GMP Human T Cell Media (Catalog # CCM038-GMP-1L) with 10 ng/mL of Recombinant Human IL-7 GMP Protein (Catalog # BT-007-GMP), 10 ng/mL Recombinant Human IL-15 GMP Protein (Catalog # BT-015-GMP) and 5% GMP human AB serum (Catalog # HABS001-GMP-100ML). Activated T cells were left untreated (No TD) or transduced with Lentivirus (TD) to express and eGFP. After 8 total days in culture, cells were assessed for expression of exhaustion marker PD-1. Data is the average of 5 T cell donors with error bars ± SD.

T cell viability and growth are equivalent in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) cytokines. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), cell density (B), and fold expansion (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype is maintained in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-7 (BT-007-GMP) and IL-15 (BT-015-GMP) cytokines. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. Frequency of CD4+ or CD8+ T cells (A), CD4+ T cell phenotype (B), and CD8+ phenotype (C) after 7 days in culture are shown. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell viability and growth are equivalent in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-2 (BT-002-GMP) cytokine. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. T cell viability (A), cells density (B), and fold expansion (C) after 7 days in culture are shown. Data is the average of 3 purified T cell donors with error bars ± SD.

T cell phenotype is maintained in fresh and pre-made media stored at 4 °C for at least 5 weeks. Xeno-Free GMP Human T Cell Media (CCM038-GMP-1L) was formulated with 5% human AB Serum (HABS001-GMP-100ML) and 10 ng/mL of IL-2 (BT-002-GMP) cytokine. Media was either used fresh or stored at 2-8 °C for 7-35 days prior to use in human T cell culture. T cells were expanded with a T cell activator in a G-Rex 6M bioreactor for 7 days. Frequency of CD4+ or CD8+ T cells (A), CD4+ T cell phenotype (B), and CD8+ phenotype (C) after 7 days in culture are shown. T cell phenotype is gated on CD45RA and CCR7. Data is the average of 3 T cell donors with error bars ± SD.