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Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear

Reduced Growth Factor Basement Membrane Extract

Key Product Details

Features: Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion. It is ideal for cultures that require limited reduced growth factor conditions.
Key Benefits:
  • Ideal for general cell culture application
  • Reduced growth factor formulation provides a more defined culture system
  • Polymerizes at 37°C to form a reconstituted basement membrane
  • Quality controlled for peformance consistency
Assessment of Pluripotency on Cultrex RGF BME.
(2)
Cultrex RGF Basement Membrane Extract_3433-010-01
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3433-010-01
3433-001-01
3433-005-01

Assay Procedure

Citations for Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear (31)

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Customer Reviews (7)

4.7 out of 5
7 customer ratings
5 stars
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4 stars
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  • Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear
    Name: Anonymous
    Verified Customer | Posted 02/21/2022
    Tube formation of Human Coronary Artery Endothelial cells imaged 6 hours after seeding.
    Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear Serum 3433-010-01
  • Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear
    Name: Anonymous
    Verified Customer | Posted 12/02/2020
  • Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear
    Name: Anonymous
    Verified Customer | Posted 03/09/2020
    Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear Serum 3433-010-01
  • Cultrex RGF Basement Membrane Extract
    Name: Anonymous
    Application: In vivo study
    Verified Customer | Posted 01/13/2020
  • Cultrex RGF Basement Membrane Extract
    Name: Anonymous
    Application: In vivo study
    Reason for Rating: I used Cultrex for an xenograft tumor growth Invivo study. I mixed Breast Cancer cells and cultrex in an 1:1 ratio and injected into mice and followed tumor growth. Cultrex helped to hold the cells together and which helped with the tumor growth.
    Verified Customer | Posted 05/27/2019
    Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear In vivo study 3433-010-01
  • Cultrex RGF Basement Membrane Extract
    Name: Anonymous
    Application: In vivo study
    Verified Customer | Posted 05/27/2019
  • Cultrex RGF Basement Membrane Extract
    Name: Anonymous
    Application: Stem cell culture and maintenance
    Verified Customer | Posted 02/08/2019
    Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear Stem cell culture and maintenance 3433-010-01

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FAQs

  • What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

    Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

  • How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

    All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

  • How should Cultrex Basement Membrane Extract (BME) be stored and handled?

    Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

  • What is the Tube Formation Assay?

    The Tube Formation Assay is based on the ability of endothelial cells to form three-dimensional capillary-like tubular structures when cultured on a hydrogel of reconstituted basement membrane, such as Cultrex Basement Membrane Extract (BME).

  • What are the advantages of the Tube Formation Assay?

    The Tube Formation Assay is the most widely used in vitro angiogenesis assay. The assay is rapid, inexpensive and quantifiable. It can be used to identify potentially angiogenic and anti-angiogenic factors, to determine endothelial cell phenotype, and to study pathways and mechanisms involved in angiogenesis. It can be performed in a high throughput mode to screen for a large number of compounds.

  • What cell types can be used in the Tube Formation Assay?

    The Tube Formation Assay is specific for endothelial cells, either primary cells or immortalized cell lines. Only endothelial cells form capillary-like structures with a lumen inside. Other endothelial cell types form other structures.

  • What are the variables associated with the Tube Formation Assay?

    The major variables associated with tube formation are composition of the Cultrex Basement Membrane Extract (BME) hydrogel, thickness of the hydrogel, cell density, composition of angiogenic factors in the assay medium, and assay period.

  • Which Cultrex Basement Membrane Extract (BME) should I use for the Tube Formation Assay?

    Cultrex Reduced Growth Factor BME (RGF BME) is generally used for testing compounds that promote angiogenesis because formation of capillary-like structures (tubes) is significantly less compared to non-growth factor reduced varieties of Cultrex BME. The Cultrex In Vitro Angiogeneis Assay (Tube Formation) includes a qualified production lot of Cultrex RGF BME that exhibits reduced background tube formation in the absence of angiogenic factors.

  • How do I reduce spontaneous formation of tubular structures on Cultrex BME in the absence of angiogenic factors?

    Primary endothelial cells, such as Human Umbilical Vein Endothelial Cells (HUVECs) form capillary-like structures in the absence of added angiogenic factors less often than immortalized endothelial cells. Generally, reducing the number of cells per cm2 plated onto Cultrex BME will result in less background or spontaneous tube formation. Titrate the number of cells and find optimal conditions for your specific cell line. When endothelial cells fully form capillary structures in response to angiogenic activators, but not in their absence, you may proceed.

  • Can cells grown in Basement Membrane Extract (BME), such as Catalog # 3433-005-01, be used in Flow Cytometry? 

    For preparation of a single cell suspension for applications such as FACS and re-culturing, digestion of the matrix and cell-cell bonds may be facilitated using enzymes such as Trypsin or Dispase. Our CellSperse, cat# 3450-048-05, is similar to Dispase, and can also be used for this purpose. However, some markers may be susceptible to degradation from CellSperse treatment. It is recommended to consult the literature for design of experiments for the particular cell type and markers of interest.

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