Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear, with Phenol Red

R&D Systems, Inc. a Bio-Techne Brand

Reduced Growth Factor Basement Membrane Extract

Key Product Details

Features: Cultrex Reduced Growth Factor Basement Membrane Extract (RGF BME) is an extracellular matrix hydrogel that has been used extensively in general cell culture applications to study cell attachment, proliferation, and invasion. It is ideal for cultures that require limited reduced growth factor conditions.
Key Benefits:
  • Ideal for general cell culture application
  • Reduced growth factor formulation provides a more defined culture system
  • Polymerizes at 37°C to form a reconstituted basement membrane
  • Quality controlled for peformance consistency
Cultrex RGF Basement Membrane Extract (with Phenol Red)
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Availability
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3433-010-01P
3433-005-01P
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Setting the standard in quality research reagents
  • Preparation & Storage

    • Preparation & Storage

    Stability Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
    Shipping Conditions The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
    Storage Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • FAQs

    • Citations for Cultrex Reduced Growth Factor Basement Membrane Extract, PathClear, with Phenol Red


    Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

    FAQs

    • What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

      Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

    • How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

      All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

    • How should Cultrex Basement Membrane Extract (BME) be stored and handled?

      Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

    Product Documents

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

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