Cultrex 3-D Culture Matrix Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL. This material is then incorporated in a 3-D culture to validate efficacy.Cultrex 3-D Culture Matrix Collagen I is a purified stromal ECM protein that has been developed, produced and qualified specifically for use in 3-D culture studies. 3-D Culture Matrix Collagen I may also be used to supplement customized hydrogel or medium formulations for cell culture.
Negative for the presence of bacteria and fungi.
