PARP/Apoptosis Chemiluminescent Assay Kit
R&D Systems, part of Bio-Techne | Catalog # 4685-096-K
For the measurment of PARP-1 activity in cell extracts
Discontinued Product
4685-096-K has been discontinued.
View all PARP products.
Key Product Details
Features
Ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.
Key Benefits
- Chemiluminescent readout
- 96 well format
- Highly sensitive – detects 0.1 mU PARP ~500 cells
- Dynamic range between 0.1 to 10 mU PARP
- Requires 10-100 ng extract for detection
- Assay Time ~3 hrs
Species
Multi-Species
Product Summary for PARP/Apoptosis Chemiluminescent Assay Kit
Why Use the PARP/Apoptosis Chemiluminescent Assay Kit?
This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity. During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.
Product Specifications
- Measure PARP-1 activity in cell extracts before and after apoptosis.
- For the screening of candidate PARP-1 inhibitors and determination of IC50 values.
Kit Contents for PARP/Apoptosis Chemiluminescent Assay Kit
- 10X Activated DNA
- PARP-HSA, 10 mUnits/µl
- 20 mM NAD
- Anti-PAR monoclonal Antibody
- Goat anti-mouse IgG-HRP
- 10 mM Etoposide
- 20X I-PAR Assay Buffer
- PARP PeroxyGlow A
- PARP PeroxyGlow™ B
- 5X Antibody Diluent
- Histone-Coated White Strip Well Plate, I-PAR
Formulation, Preparation, and Storage
Shipping
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: PARP
PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
Long Name
Poly [ADP-ribose] Polymerase
Alternate Names
ADPRT, PARP1, PPOL
Additional PARP Products
Product Documents for PARP/Apoptosis Chemiluminescent Assay Kit
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