Proteome Profiler Human XL Cytokine Array Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # ARY022B
Contains 4 membranes - each spotted in duplicate with 105 different cytokine antibodies
Key Product Details
Species
Human
Product Summary for Proteome Profiler Human XL Cytokine Array Kit
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.
Key Benefits
- Detects 105 human cytokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Array
The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Human XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
|---|---|---|
| Adiponectin/Acrp30 | IFN-gamma | CCL2/MCP-1 |
| Angiogenin | IGFBP-2 | CCL7/MCP-3 |
| Angiopoietin-1 | IGFBP-3 | M-CSF |
| Angiopoietin-2 | IL-1 alpha/IL-1F1 | MIF |
| Apolipoprotein A1 | IL-1 beta/IL-1F2 | CXCL9/MIG |
| BAFF/BLyS/TNFSF13B | IL-1ra/IL-1F3 | CCL3/CCL4 MIP-1 alpha/beta |
| BDNF | IL-2 | CCL20/MIP-3 alpha |
| CD14 | IL-3 | CCL19/MIP-3 beta |
| CD30 | IL-4 | MMP-9 |
| CD31/PECAM-1 | IL-5 | Myeloperoxidase |
| CD40 Ligand/TNFSF5 | IL-6 | Osteopontin (OPN) |
| Chitinase 3-like | IL-8 | PDGF-AA |
| Complement Component C5/C5a | IL-10 | PDGF-AB/BB |
| Complement Factor D | IL-11 | Pentraxin 3/TSF-14 |
| C-Reactive Protein/CRP | IL-12 p70 | CXCL4/PF4 |
| Cripto-1 | IL-13 | RAGE |
| Cystatin C | IL-15 | CCL5/RANTES |
| Dkk-1 | IL-16 | RBP4 |
| DPPIV/CD26 | IL-17A | Relaxin-2 |
| EGF | IL-18 BPa | Resistin |
| CXCL5/ENA-78 | IL-19 | CXCL12/SDF-1 alpha |
| Endoglin/CD105 | IL-22 | Serpin E1/PAI-1 |
| EMMPRIN | IL-23 | SHBG |
| Fas Ligand | IL-24 | ST2/IL1 R4 |
| FGF basic | IL-27 | CCL17/TARC |
| KGF/FGF-7 | IL-31 | TFF3 |
| FGF-19 | IL-32 alpha/beta/gamma | TfR |
| Flt-3 Ligand | IL-33 | TGF-alpha |
| G-CSF | IL-34 | Thrombospondin-1 |
| GDF-15 | CXCL10/IP-10 | TIM-3 |
| GM-CSF | CXCL11/I-TAC | TNF-alpha |
| CXCL1/GRO alpha | Kallikrein 3/PSA | uPAR |
| Growth Hormone (GH) | Leptin | VCAM-1 |
| HGF | LIF | VEGF |
| ICAM-1/CD54 | Lipocalin-2/NGAL | Vitamin D BP |
Scientific Data Images for Proteome Profiler Human XL Cytokine Array Kit
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Multiplex Cytokine Assay Kits
Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Proteome Profiler Human XL Cytokine Array Kit
Product Specific Notices for Proteome Profiler Human XL Cytokine Array Kit
For research use only
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